Use of copper cast alloys to control Escherichia coli O157 cross-contamination during food processing
Use of copper cast alloys to control Escherichia coli O157 cross-contamination during food processing
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DOI：
10.1128/aem.02532-05
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发表时间：
2006-06-01
影响因子：
4.4
通讯作者：
Keevil, C. W.
中科院分区：
文献类型：
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作者：
Noyce, J. O.;Michels, H.;Keevil, C. W.
大肠杆菌O 157（E.大肠杆菌O 157）是通过受污染的食品，通常是碎牛肉。本研究的目的是评估七种铸造铜合金（61 - 95% Cu）降低大肠杆菌存活率的能力。大肠杆菌O 157，与或没有碎牛肉汁混合，并比较这些结果与不锈钢。E.将与提取的牛肉汁（25%）混合的大肠杆菌O 157（NCTC 12900）（2 × 10（7）CFU）接种到每种铜铸造合金或不锈钢的试样上，并在22 ℃或4 ℃下孵育最多6小时。E.除了用呼吸指示剂荧光染料5-氰基-2，3-二甲苯基四唑原位染色外，还通过平板计数来测定大肠杆菌O 157的存活力。在没有牛肉膏的情况下，三种合金在22 ℃下暴露6小时期间完全杀死了接种物。在4摄氏度下，只有高铜合金（> 85%）显着减少了O 157的数量。对于牛肉汁，只有一种合金（95%Cu）在22 ℃下完全杀死了接种物。对于不锈钢，细胞数量没有显著减少。在4 ℃时，只有合金C83300（93% Cu）和C87300（95% Cu）显著降低了E的数量。大肠杆菌O 157，分别具有1.5和5个对数的杀灭率。将接种物减少到10（3）CFU导致在22 ℃下在20分钟或更短时间内完全杀灭所有七种铸造铜合金。这些结果清楚地证明了铸造铜合金对大肠杆菌的抗菌性能。大肠杆菌O 157，因此这些合金具有帮助食品安全的潜力。
The most notable method of infection from Escherichia coli O157 (E. coli O157) is through contaminated food products, usually ground beef. The objective of this study was to evaluate seven cast copper alloys (61 to 95% Cu) for their ability to reduce the viability of E. coli O157, mixed with or without ground beef juice, and to compare these results to those for stainless steel. E. coli O157 (NCTC 12900) (2 X 10(7) CFU) mixed with extracted beef juice (25%) was inoculated onto coupons of each copper cast alloy or stainless steel and incubated at either 22 degrees C or 4 degrees C for up to 6 h. E. coli O157 viability was determined by plate counts in addition to staining in situ with the respiratory indicator fluorochrome 5-cyano-2,3-ditolyl tetrazolium. Without beef extract, three alloys completely killed the inoculum during the 6-h exposure at 22 degrees C. At 4 degrees C, only the high-copper alloys (> 85%) significantly reduced the numbers of O157. With beef juice, only one alloy (95% Cu) completely killed the inoculum at 22 degrees C. For stainless steel, no significant reduction in cell numbers occurred. At 4 degrees C, only alloys C83300 (93% Cu) and C87300 (95% Cu) significantly reduced the numbers of E. coli O157, with 1.5- and 5-log kills, respectively. Reducing the inoculum to 10(3) CFU resulted in a complete kill for all seven cast copper alloys in 20 min or less at 22 degrees C. These results clearly demonstrate the antimicrobial properties of cast copper alloys with regard to E. coli O157, and consequently these alloys have the potential to aid in food safety.