Comparative transcriptional profiling of the axolotl limb identifies a tripartite regeneration-specific gene program.
Comparative transcriptional profiling of the axolotl limb identifies a tripartite regeneration-specific gene program.
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DOI：
10.1371/journal.pone.0061352
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Tanaka EM
中科院分区：
文献类型：
--
作者：
Knapp D;Schulz H;Rascon CA;Volkmer M;Scholz J;Nacu E;Le M;Novozhilov S;Tazaki A;Protze S;Jacob T;Hubner N;Habermann B;Tanaka EM
了解肢体成纤维细胞是如何在最初的伤口愈合反应后建立的，是再生研究的一个重要方面。在这里，我们对Axolotl的侧方伤口愈合和截肢的时间进程进行了平行表达谱分析。这种伤口愈合和再生之间的比较使我们能够识别截肢特异性基因。通过对这些样本的表达谱进行聚类，我们可以检测到三个明显的基因表达阶段-早期伤口愈合，然后是导致肢体发育程序建立的过渡阶段，这三个阶段对应于形态标准定义的肢体再生的三个阶段。通过对过渡期的研究，我们确定了93个严格截肢相关的基因，其中许多与氧化应激反应、染色质修饰、上皮发育或肢体发育有关。我们进一步根据这些基因是否在发育中的肢芽中显著表达对它们进行了分类。用原位杂交的方法研究了53个候选基因在胚泡内的特异性定位。综上所述，我们确定了一组在再生过程中特异表达的基因，因此可能是调控胚泡形成的候选基因。
Understanding how the limb blastema is established after the initial wound healing response is an important aspect of regeneration research. Here we performed parallel expression profile time courses of healing lateral wounds versus amputated limbs in axolotl. This comparison between wound healing and regeneration allowed us to identify amputation-specific genes. By clustering the expression profiles of these samples, we could detect three distinguishable phases of gene expression – early wound healing followed by a transition-phase leading to establishment of the limb development program, which correspond to the three phases of limb regeneration that had been defined by morphological criteria. By focusing on the transition-phase, we identified 93 strictly amputation-associated genes many of which are implicated in oxidative-stress response, chromatin modification, epithelial development or limb development. We further classified the genes based on whether they were or were not significantly expressed in the developing limb bud. The specific localization of 53 selected candidates within the blastema was investigated by in situ hybridization. In summary, we identified a set of genes that are expressed specifically during regeneration and are therefore, likely candidates for the regulation of blastema formation.
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影响因子：
64.5
作者：
An, W;Kim, J;Roeder, RG
通讯作者：
Roeder, RG
影响因子：
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作者：
Guimond JC;Lévesque M;Michaud PL;Berdugo J;Finnson K;Philip A;Roy S
通讯作者：
Roy S
影响因子：
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作者：
Endo, T;Bryant, SV;Gardiner, DM
通讯作者：
Gardiner, DM
影响因子：
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通讯作者：
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影响因子：
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作者：
Elsaesser, Simon J.;Goldberg, Aaron D.;Allis, C. David
通讯作者：
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