Lectin receptors of rods and cones. Visualization by fluorescent label.
Lectin receptors of rods and cones. Visualization by fluorescent label.
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DOI：
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发表时间：
1981
影响因子：
4.4
通讯作者：
C. Bridges
中科院分区：
文献类型：
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作者：
C. Bridges
研究了8种荧光标记凝集素与视觉细胞的结合情况。刀豆蛋白A(ConA)(专用于含有甘露糖的寡糖)均匀地结合到青蛙、牛、金鱼和海龟的杆状外节(ROS)的表面。在蛙类中，结合在内节上观察到，内/外节段交界处和核区的结合增强。在ROS盘的表面上没有结合。在0.1%的Triton X-100中，棒状物形成缠绕的管状结构，将ConA结合到它们的表面。分离的锥体外段(COSS)与内段相比显示出均匀的荧光，内段仅显示表面标记。只有与岩藻糖(UEA、LTA)有亲和力的凝集素不能与ROSS和COS结合。与含半乳糖的低聚糖(RCA-120、RCA-60和PNA)有亲和力的凝集素几乎不能与杆状内段结合。WGA(N-乙酰氨基葡萄糖)和SBA(N-乙酰氨基半乳糖和半乳糖)都不分青红皂白地与所有ROS结合，而PNA优先与附生球果结合。
The binding of eight fluorescence-labeled lectins to visual cells was investigated. Concanavalin A (Con A) (specific for oligosaccharides containing mannose) bound evenly to the surfaces of rod outer segments (ROSs) from frog, cattle, goldfish, and turtle (Pseudemys). In frog, binding was observed on the inner segment, with intensifications at the inner/outer segment junction and in the region of the nucleus. No binding occurred on the surfaces of ROS discs. In 0.1% Triton X-100, rods formed convoluted tubular structures that bound Con A to their surfaces. Isolated cone outer segments (COSs) showed uniform fluorescence as compared with the inner segments, which displayed only surface labeling. Only lectins with an affinity for fucose (UeA, LTA) failed to bind to the ROSs and COS. Lectins with an affinity for oligosaccharides containing galactose (RCA-120, RCA-60, and PNA) bound hardly at all to the rod inner segments. Both WGA (N-acetyl glucosamine) and SBA (N-acetyl galactosamine and galactose) bound indiscriminately to all ROSs, whereas PNA bound preferentially to the accessory cones.