Apoptosis of lung carcinoma cells induced by a flexible optical fiber-based cold microplasma
Apoptosis of lung carcinoma cells induced by a flexible optical fiber-based cold microplasma
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DOI：
10.1016/j.bios.2011.07.039
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发表时间：
2011-10-15
影响因子：
12.6
通讯作者：
Kim, Sung-O.
中科院分区：
文献类型：
--
作者：
Kim, Jae Young;Ballato, John;Kim, Sung-O.
大气压等离子体已被用作癌症的治疗。然而，目前的等离子体递送系统的相当大的尺寸和刚度阻碍了对难以到达的内脏器官（例如肺、胰腺和十二指肠）中的肿瘤的精确治疗。为了改善等离子体的靶向输送，使用内径为15 μ m、55 μ m或200 μ m的空芯光纤制造高度柔性的微等离子体射流装置。基于该装置，本文描述了使用微等离子体癌症内窥镜进行肺癌治疗的结果。尽管小的内径和低的气体流速，所产生的等离子体射流被证明是足够有效的诱导细胞凋亡，但不坏死，在培养的小鼠肺癌和成纤维细胞。此外，基于总体血浆剂量条件，发现肺癌细胞比成纤维细胞对血浆处理更敏感。这项工作使得能够使用高度灵活和精确的基于中空光纤的等离子体设备进行定向癌症治疗，并使用改进的等离子体靶向和递送方法增强微等离子体癌症内窥镜检查。(C)2011爱思唯尔有限公司版权所有。
Atmospheric pressure plasmas have been used as a therapy for cancer. However, the fairly large size and rigidity of present plasma-delivery systems obstructs the precise treatment of tumors in harder-to-reach internal organs such as the lungs, pancreas, and duodenum. In order to improve the targeted delivery of plasmas a highly flexible microplasma jet device is fabricated using a hollow-core optical fiber with an inner diameter of either 15 mu m, 55 mu m, or 200 mu m. Described herein, based on this device, are results on lung carcinoma therapy using a microplasma cancer endoscope. Despite the small inner diameter and the low gas flow rate, the generated plasma jets are shown to be sufficiently effective to induce apoptosis, but not necrosis, in both cultured mouse lung carcinoma and fibroblast cells. Further, the lung carcinoma cells were found to be more sensitive to plasma treatment than the fibroblast cells based on the overall plasma dose conditions. This work enables directed cancer therapies using on highly flexible and precise hollow optical fiber-based plasma device and offers enhancements to microplasma cancer endoscopy using an improved method of plasma targeting and delivery. (C) 2011 Elsevier B.V. All rights reserved.