The Tomato MADS-Box Transcription Factor RIPENING INHIBITOR Interacts with Promoters Involved in Numerous Ripening Processes in a COLORLESS NONRIPENING-Dependent Manner
The Tomato MADS-Box Transcription Factor RIPENING INHIBITOR Interacts with Promoters Involved in Numerous Ripening Processes in a COLORLESS NONRIPENING-Dependent Manner
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DOI：
10.1104/pp.111.181107
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发表时间：
2011-11-01
期刊：
影响因子：
7.4
通讯作者：
Giovannoni, James J.
中科院分区：
文献类型：
--
作者：
Martel, Catherine;Vrebalov, Julia;Giovannoni, James J.
果实成熟是一个复杂的发育过程，负责将含种子的器官转化为对种子传播者和农业消费者有吸引力的组织。实现这种变化所需的不同生化途径的协调调节受到了相当大的研究关注。MADS盒转录因子RIPENING INHIBITOR（RIN）是番茄果实成熟的重要调控因子，但其影响成熟相关基因表达的确切机制尚不清楚。使用染色质免疫沉淀方法，我们提供的证据表明，RIN与参与主要途径的基因启动子相互作用，这些途径与观察到的和充分研究的成熟表型和现象相关，包括参与整体成熟调控的转录控制网络，乙烯生物合成，乙烯感知，下游乙烯反应，细胞壁代谢和类胡萝卜素生物合成。此外，在乙烯和类胡萝卜素生物合成的情况下，RIN与编码限速活性的基因的启动子相互作用。我们还表明，RIN招聘的目标基因座是依赖于一个正常运作的等位基因在成熟特异性转录因子COLORLESS NONRIPENING基因座，进一步澄清这两个成熟调节剂之间的关系。
Fruit ripening is a complex developmental process responsible for the transformation of the seed-containing organ into a tissue attractive to seed dispersers and agricultural consumers. The coordinated regulation of the different biochemical pathways necessary to achieve this change receives considerable research attention. The MADS-box transcription factor RIPENING INHIBITOR (RIN) is an essential regulator of tomato (Solanum lycopersicum) fruit ripening but the exact mechanism by which it influences the expression of ripening-related genes remains unclear. Using a chromatin immunoprecipitation approach, we provide evidence that RIN interacts with the promoters of genes involved in the major pathways associated with observed and well-studied ripening phenotypes and phenomena, including the transcriptional control network involved in overall ripening regulation, ethylene biosynthesis, ethylene perception, downstream ethylene response, cell wall metabolism, and carotenoid biosynthesis. Furthermore, in the cases of ethylene and carotenoid biosynthesis, RIN interacts with the promoters of genes encoding rate-limiting activities. We also show that RIN recruitment to target loci is dependent on a normally functioning allele at the ripening-specific transcription factor COLORLESS NONRIPENING gene locus, further clarifying the relationship between these two ripening regulators.