Echinoid Regulates Tracheal Morphology and Fusion Cell Fate in Drosophila
Echinoid Regulates Tracheal Morphology and Fusion Cell Fate in Drosophila
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DOI：
10.1002/dvdy.22386
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发表时间：
2010-09-01
影响因子：
2.5
通讯作者：
Nilson, Laura A.
中科院分区：
文献类型：
--
作者：
Laplante, Caroline;Paul, Sarah M.;Nilson, Laura A.
果蝇胚胎气管的形态发生涉及上皮管分支和融合的定型模式。在这里，我们报告意外的表型导致的母体和合子（M/Z）的同源细胞粘附分子棘（艾德）的损失，以及亚细胞定位的艾德在气管。艾德（M/Z）胚胎具有令人联想到分隔连接（SJ）和管腔基质突变体的卷曲气管。然而，艾德不定位于SJS，并且ECE胚胎具有完整的SJS，并且显示基质修饰蛋白蠕虫状的正常管腔聚集。令人惊讶的是，气管长度并没有增加edmm突变体，但以前未描述的组合减少节间间距和深表皮沟产生一个回旋气管表型。此外，edmm突变体具有独特的融合缺陷，涉及额外融合细胞、异位融合事件和非典型分支断裂。气管特异性表达的艾德挽救这些融合缺陷，表明艾德在气管中的作用，以控制融合细胞的命运。Developmental Dynamics 239：2509-2519，2010. (C)2010 Wiley-Liss，Inc.
Morphogenesis of the Drosophila embryonic trachea involves a stereotyped pattern of epithelial tube branching and fusion. Here, we report unexpected phenotypes resulting from maternal and zygotic (M/Z) loss of the homophilic cell adhesion molecule Echinoid (Ed), as well as the subcellular localization of Ed in the trachea. ed(M/Z) embryos have convoluted trachea reminiscent of septate junction (SJ) and luminal matrix mutants. However, Ed does not localize to SJs, and ece" embryos have intact SJs and show normal luminal arxumulation of the matrix-modifying protein Vermiform. Surprisingly, tracheal length is not increased in edmm mutants, but a previously undescribed combination of reduced intersegmental spacing and deep epidermal grooves produces a convoluted tracheal phenotype. In addition, edmm mutants have unique fu ion defects involving supernumerary fusion cells, ectopic fusion events and atypical branch breaks. Tracheal-specific expression of Ed rescues these fusion defects, indicating that Ed acts in trachea to control fusion cell fate. Developmental Dynamics 239:2509-2519, 2010. (C) 2010 Wiley-Liss, Inc.