Mycorrhization helper bacteria: a case of specificity for altering ectomycorrhiza architecture but not ectomycorrhiza formation
Mycorrhization helper bacteria: a case of specificity for altering ectomycorrhiza architecture but not ectomycorrhiza formation
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DOI：
10.1007/s00572-006-0068-3
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发表时间：
2006-11-01
期刊：
影响因子：
3.9
通讯作者：
Bending, Gary D.
中科院分区：
文献类型：
--
作者：
Aspray, Thomas J.;Frey-Klett, Pascale;Bending, Gary D.
从系统发育不同的外生菌根共生体中分离到的菌丝化辅助细菌(MHB)，包括乳菇、双色乳菇或黄乳杆菌，进行了真菌专一性试验，以增强红松或双色乳杆菌的菌根共生体。西尔维斯特菌根形成。由于从L.Rufus和S.luteus菌根中分离的MHB最初是使用微型系统表征的，因此我们评估了它们在温室系统中促进菌根形成的能力，以确定MHB具有系统特异性的程度。拟芽孢杆菌属微观世界中Rufus的一种MHB EJP73显著促进了温室中双色乳杆菌菌根的形成，表明该细菌的MHB效应既不是真菌特异性的，也不限于原始实验系统。虽然所研究的5株MHB菌株不能显著促进红曲霉菌根的形成，但其中两株MHB菌株对红曲霉的二叉短根分枝有显著的影响。对拟青霉属细菌有明显的抑制作用。EJP73和Burkholderia sp.EJP67，这两株菌株都是从红曲霉菌根中分离出来的，与生长素的产生无关。菌根结构的改变，而不是菌根的绝对数量，可能是定义MHB效应的一个以前被忽视的重要参数。
Mycorrhization helper bacteria (MHB), isolated from phylogenetically distinct ectomycorrhizal symbioses involving Lactarius rufus, Laccaria bicolor or Suillus luteus, were tested for fungus specificity to enhance L. rufus-Pinus sylvestris or L. bicolor-P. sylvestris mycorrhiza formation. As MHB isolated from the L. rufus and S. luteus mycorrhiza were originally characterised using a microcosm system, we assessed their ability to enhance mycorrhiza formation in a glasshouse system in order to determine the extent to which MHB are system-specific. Paenibacillus sp. EJP73, an MHB for L. rufus in the microcosm, significantly enhanced L. bicolor mycorrhiza formation in the glasshouse, demonstrating that the MHB effect of this bacterium is neither fungus-specific nor limited to the original experimental system. Although the five MHB strains studied were unable to significantly enhance L. rufus mycorrhiza formation, two of them did have a significant effect on dichotomous short root branching by L. rufus. The effect was specific to Paenibacillus sp. EJP73 and Burkholderia sp. EJP67, the two strains isolated from L. rufus mycorrhiza, and was not associated with auxin production. Altered mycorrhiza architecture rather than absolute number of mycorrhizal roots may be an important previously overlooked parameter for defining MHB effects.