Construction of a genetic linkage map and QTL mapping for sex indicate the putative genetic pattern of the F1 hybrid Scylla (Scylla serrata 9 x S. paramamosain cl)
Construction of a genetic linkage map and QTL mapping for sex indicate the putative genetic pattern of the F1 hybrid Scylla (Scylla serrata 9 x S. paramamosain cl)
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遗传连锁图谱的构建和性别 QTL 定位表明了 F1 代杂交青蟹 (Scylla serrata â à S. paramamosain â) 的假定遗传模式
DOI：
10.1016/j.aquaculture.2021.737222
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发表时间：
2021-07-30
期刊：
影响因子：
4.5
通讯作者：
Ma, Hongyu
中科院分区：
文献类型：
--
作者：
Cui, Wenxiao;Guan, Mengyun;Ma, Hongyu
为了为阐明F1杂种青蟹（Scylla serrata 9 x S. paramamosain cl）基因组基因组序列组装提供有价值的框架，并为QTL定位和标记辅助选择提供参考，本文获得了F1杂种后代并用于构建第一个杂种青蟹遗传连锁图谱。我们提出了一个性别平均遗传连锁图，其中包含 49 个连锁群 (LG)，涵盖 5574 个 SNP 标记。男性地图由 40 个 LG 组成，而女性地图由 22 个 LG 组成。该遗传连锁图谱的高质量通过扭曲标记比例低、每个LG上相邻标记之间的强连锁关系以及每个个体中较大片段的一致来源得到验证。分布在3个QTL区域的性连锁标记均位于LG12上，表明LG12对应的染色体似乎是F1杂种后代的性染色体。对 F1 杂交后代和 S. paramamosain 之间的遗传图谱进行性别连锁 LG 比较，表明 F1 杂交后代中 ZW/ZZ 性别决定系统的构象。基于该遗传连锁图的高质量，我们描述了 F1 杂种二倍体后代的假定遗传模式。假定的遗传模式主张不同物种之间染色体突触的形成可能是染色体形式分歧的最初诱因。
To provide a valuable framework for genome sequence assembly towards the elucidation of the F1 hybrid Scylla (Scylla serrata 9 x S. paramamosain cl) genome as well as a reference for QTL mapping and maker-assisted selection, herein F1 hybrid offspring were obtained and used to construct the first genetic linkage map of hybrid Scylla. We presented a sex-averaged genetic linkage map with 49 linkage groups (LGs) covering 5574 SNP markers. The male map consisted of 40 LGs, whereas the female map comprises 22 LGs. The high quality of this genetic linkage map was verified by the low proportion of distorted markers, the strong linkage relationship between adjacent markers on each LG, and the consistent source of the larger segment in each individual. All sexlinked markers distributed in three QTL regions were located on LG12, indicating the chromosome corresponding to LG12 seems to be the sex chromosome of the F1 hybrid offspring. A sex-linked LG comparison of the genetic maps between F1 hybrid offspring and the S. paramamosain suggested the conformation of a ZW/ZZ sex determination system in F1 hybrid offspring. Based on the high quality of this genetic linkage map, we described the putative genetic pattern of F1 hybrid diploid offspring. The putative genetic pattern argues the proposition that a formation of chromosome synapsis among different species might be an initial inducement for the divergence of chromosomal forms.