The in vitro effects of metal cations on eukaryotic cell metabolism.
The in vitro effects of metal cations on eukaryotic cell metabolism.
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金属阳离子对真核细胞代谢的体外影响。
DOI：
10.1002/jbm.820250907
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Craig,RG
中科院分区：
文献类型：
--
作者：
Wataha,JC;Hanks,CT;Craig,RG
使用Balb/c 3T3成纤维细胞和四个毒性参数评估牙科铸造合金中常见的九种金属阳离子的体外细胞毒性：总蛋白质产生、3H-亮氨酸掺入、3H-胸苷掺入和MTT-甲瓒产生。测定了与对照组相比引起50%毒性的浓度（TC 50）和这些效应的可逆性。金属阳离子的效力范围为2 - 3个数量级，其中Cd 2+表现出最大效力，In 3+表现出最小效力。效价与这些金属的原子量无关。对于每种金属阳离子，在大多数情况下，各种毒性参数的TC 50是相似的。然而，几种阳离子（Cu2+，Ga3+）显示出更大的效力与3H-胸苷掺入。观察到所有阳离子的毒性效应都是可逆的;在每种阳离子的TC 50值范围内的浓度下，这些效应通常变得不可逆。几个刺激效果被看到。小，但统计学上显着的刺激后，观察到金属暴露24小时的Ag 1+，Au 4+，Cu 2+，Ga 3+，和Ni 2+。剩余的刺激24小时后，去除金属阳离子，观察到Au 4+，Cd 2+，Ni 2+，和Zn 2+。刺激总是在低于TC 50浓度的浓度下发生。本研究可用于评价牙科合金释放的金属阳离子的潜在细胞毒性作用。
Thein vitrocytotoxicity of nine metal cations common in dental casting alloys was evaluated using Balb/c 3T3 fibroblasts and four toxicity parameters: total protein production,3H‐leucine incorporation,3H‐thymidine incorporation, and MTT‐formazan production. Concentrations causing 50% toxicity compared to controls (TC50's) and reversibility of these effects were determined. The range of potency of the metal cations was 2–3 orders of magnitude, with Cd2+showing the greatest potency and In3+showing the least. Potency did not correlate with atomic weight for these metals. For each metal cation, the TC50's of the various toxicity parameters were similar in most cases. However, several cations (Cu2+, Ga3+) showed greater potency with3H‐thymidine incorporation. Reversibility of the toxic effects was observed for all cations; the effects generally became irreversible at concentrations in the range of the TC50 value for each cation. Several stimulatory effects were seen. Small but statistically significant stimulations were observed after 24 h of metal exposure for Ag1+, Au4+, Cu2+, Ga3+, and Ni2+. Residual stimulations 24 h after removal of the metal cations were observed for Au4+, Cd2+, Ni2+, and Zn2+. Stimulations always occurred at concentrations below the TC50 concentrations. This study should be useful in evaluating the potential cytotoxic effects of metal cations released from dental alloys.