A human embryonic stem cell-based in vitro model revealed that ultrafine carbon particles may cause skin inflammation and psoriasis
A human embryonic stem cell-based in vitro model revealed that ultrafine carbon particles may cause skin inflammation and psoriasis
复制标题
DOI：
10.1016/j.jes.2019.06.016
复制
发表时间：
2020-01-01
影响因子：
6.9
通讯作者：
Faiola, Francesco
中科院分区：
文献类型：
--
作者：
Cheng, Zhanwen;Liang, Xiaoxing;Faiola, Francesco
空气污染与许多健康问题有关，包括皮肤问题，特别是儿童。在所有的大气污染物中，超细颗粒物被认为是非常危险的，因为它们很容易穿透肺部和皮肤，并被吸收到血液中。在这里，我们使用了基于人类胚胎干细胞(HESC)的角质形成细胞分化系统，以测试与环境相关的浓度下超细碳颗粒(模拟环境中的超细颗粒)的效果。我们发现，10 ng/mL10微克/毫升的超细碳颗粒下调了多能标记SOX2在人胚胎干细胞中的表达。1~10mU g/mLC颗粒处理组角质形成细胞分化受阻，IL-1β、IL-6、CXCL1、CXCL2、CXCL3、CCL20、CXCL8、S100A7、S100A9等炎症和银屑病相关基因表达上调。总体而言，我们的结果为大气超细颗粒物的潜在发育毒性提供了新的见解。(C)2019年中国科学院生态环境科学研究中心。爱思唯尔出版公司(Elsevier B.V.)
Air pollution has been linked to many health issues, including skin conditions, especially in children. Among all the atmospheric pollutants, ultrafine particles have been deemed very dangerous since they can readily penetrate the lungs and skin, and be absorbed into the bloodstream. Here, we employed a human embryonic stem cell (hESC)-based differentiation system towards keratinocytes, to test the effects of ultrafine carbon particles, which mimic ambient ultrafine particles, at environment related concentrations. We found that 10 ng/mL to 10 mu g/mL ultrafine carbon particles down-regulated the expression of the pluripotency marker SOX2 in hESCs. Moreover, 1 mu g/mL to 10 mu g/mL carbon particle treatments disrupted the keratinocyte differentiation, and up-regulated inflammation- and psoriasis-related genes, such as IL-1 beta, IL-6, CXCL1, CXCL2, CXCL3, CCL20, CXCL8, and S100A7 and S100A9, respectively. Overall, our results provide a new insight into the potential developmental toxicity of atmospheric ultrafine particles. (c) 2019 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.