Osteopontin expression in lung cancer
Osteopontin expression in lung cancer
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DOI：
10.1016/0169-5002(95)00595-1
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发表时间：
1996-11-01
期刊：
影响因子：
5.3
通讯作者：
Casson, AG
中科院分区：
文献类型：
--
作者：
Chambers, AF;Wilson, SM;Casson, AG
骨桥蛋白（Osteopontin， OPN）是一种整合素结合转化相关蛋白，由培养的肿瘤细胞系分泌，在一些实验肿瘤系统中与恶性肿瘤增加有关。然而，关于OPN表达在人类癌症中的意义，我们所知甚少。本研究的目的是确定OPN是否在一系列手术切除的肺癌中表达，以及OPN表达与临床病理表现或预后之间是否存在关系。对25例行根治性肺切除术的患者进行前瞻性研究。从每位患者的原发肿瘤和远处正常肺组织中提取RNA。采用northern印迹法检测OPN RNA水平。使用抗OPN单克隆抗体对石蜡包埋组织进行免疫组化，以评估OPN蛋白在组织中的分布。相对于配对的正常组织，OPN RNA和蛋白在大多数肿瘤中过表达。opn免疫阳性的肿瘤细胞有差异。在一些病例中，OPN存在于肿瘤细胞中，而在大多数病例中，OPN主要存在于肿瘤浸润的巨噬细胞和坏死区域。OPN RNA或蛋白的过度表达通常与临床病理结果无关。然而，肿瘤中opn免疫阳性与患者生存之间存在统计学意义上的相关性。这些发现表明，肺肿瘤中的OPN水平有可能为患者预后提供重要的临床预测信息，并且OPN可能在肺癌生物学中发挥作用。
Osteopontin (OPN), an integrin-binding, transformation-associated protein, is secreted by tumor cell lines in culture and is associated with increased malignancy in some experimental tumor systems. Little is known, however, about the significance of OPN expression in human cancers. The aims of this study were to determine if OPN was expressed in a series of surgically resected lung cancers, and if there was a relationship between OPN expression and clinico-pathologic findings or outcome. Twenty-five patients who underwent curative pulmonary resection were studied prospectively. RNA was extracted from primary tumor and distant normal lung tissue for each patient. OPN RNA levels were evaluated by northern blotting. Immunohistochemistry on paraffin-embedded tissue, using an anti-OPN monoclonal antibody, was performed to assess tissue distribution of OPN protein. OPN RNA and protein were over-expressed in the majority of tumors, relative to paired normal tissue. There was variation in the cells of the tumor that were OPN-immunopositive. In some cases OPN was present in tumor cells, while in the majority of cases OPN was detected primarily in tumor-infiltrating macrophages and necrotic areas. Over-expression of OPN RNA or protein generally was not related to clinico-pathological findings. However, there was a statistically significant association between OPN-immunopositivity in the tumor and patient survival. These findings suggest that OPN levels in lung tumors have the potential to provide clinically important predictive information on patient outcome, and that OPN may play a role in the biology of lung cancer.