The effect of antioxidants and a caspase inhibitor on cryopreserved rat hepatocytes
The effect of antioxidants and a caspase inhibitor on cryopreserved rat hepatocytes
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DOI：
10.3727/000000005783982981
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发表时间：
2005-01-01
影响因子：
3.3
通讯作者：
Demetriou, AA
中科院分区：
文献类型：
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作者：
Fujita, R;Hui, T;Demetriou, AA
肝细胞移植和使用生物人工肝支持系统已被认为是暴发性肝功能衰竭的潜在治疗方法。液氮冷冻保存是目前长期保存分离肝细胞的主要方法。然而，冷冻保存可导致低细胞回收率和分化功能的降低。已经提出了在冷冻保存期间细胞死亡的几种可能的机制。最重要的机制似乎是氧化应激和细胞凋亡。在这项研究中，我们分离新鲜大鼠肝细胞，并将其冷冻保存在三种培养基中：威斯康星州大学（UW）溶液，含抗氧化剂的培养基，和含半胱天冬酶抑制剂的培养基。检查了在这些培养基中冻存的肝细胞的活力和功能。冻存条件对解冻后肝细胞活力无影响。然而，培养后，我们发现抗氧化剂和半胱天冬酶处理的肝细胞在6和24 h的活力和功能显着改善。
Hepatocyte transplantation and use of bioartificial liver support systems have been suggested as potential therapies for fulminant hepatic failure. Cryopreservation in liquid nitrogen is presently the major method of long-term storage of isolated hepatocytes. However, cryopreservation can result in low cell recovery and reduction in differentiated function. Several possible mechanisms of cell death during cryopreservation have been proposed. The most important mechanisms appear to be oxidative stress and apoptosis. In this study, we isolated fresh rat hepatocytes and cryopreserved them in three media: University of Wisconsin (UW) solution, an anti oxidant-containing medium, and medium containing a caspase inhibitor. Viability and function of hepatocytes cryopreserved in these media were examined. Cryopreservation conditions had no effect on hepatocyte viability after thawing. However, after culture we found significant improvements in viability and function in both antioxidant- and caspase inhibitor-treated hepatocytes at 6 and 24 h.