Role of ascorbic acid in enhancing hypoxia tolerance in roots of sensitive and tolerant apple rootstocks
Role of ascorbic acid in enhancing hypoxia tolerance in roots of sensitive and tolerant apple rootstocks
复制标题
抗坏血酸在增强敏感和耐性苹果砧木根系耐缺氧能力中的作用
DOI：
10.1016/j.scienta.2013.10.003
复制
发表时间：
2013-12-17
影响因子：
4.3
通讯作者：
Ma, Fengwang
中科院分区：
文献类型：
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作者：
Bai, Tuanhui;Ma, Ping;Ma, Fengwang
抗坏血酸（阿萨）在植物生长发育中具有重要作用，在环境胁迫下保护细胞免受氧化的挑战。本研究探讨了外源性阿萨增强敏感和耐受细胞耐缺氧能力和减轻氧化损伤的机制。苹果砧木（Malus sieversii和M. hupehensis）。虽然这种压力抑制幼苗生长在这两个物种，这种影响的程度有很大的不同，两种基因型。在胁迫下，干重显著低于低氧敏感的M。而添加阿萨后，则显著增加。低氧胁迫下，两种植物根系过氧化氢（H2 O2）和超氧阴离子自由基（O-2）含量均显著增加。这导致了氧化损伤，这是由丙二醛（MDA）的积累表示。然而，M.添加阿萨可显著抑制sieversii的生长。外源施用阿萨A增强了这两个物种的抗坏血酸过氧化物酶（APX）、脱氢抗坏血酸还原酶（达尔）、单脱氢抗坏血酸还原酶（MDHAR）、谷胱甘肽还原酶（GR）、超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）的活性，但在M中的程度更大。sieversii比M.低氧胁迫下的湖北海棠外源施用阿萨提高了缺氧条件下两种苹果属植物的内源AsA水平，但在M. siversii。阿萨应用程序也略有提高根谷胱甘肽（GSH）含量在强调植物，但差异不具有统计学意义的处理和未处理的植物。阿萨可能通过提高内源性AsA的水平，改善抗坏血酸-谷胱甘肽循环能力，从而减少膜氧化损伤的发生，从而增强细胞耐缺氧能力。(C)2013爱思唯尔有限公司版权所有。
Ascorbic acid (AsA) has an important role in plant growth and development, protecting cells against oxidative challenges when exposed to environmental stresses. We investigated the mechanism by which exogenous AsA enhances hypoxia tolerance and alleviates oxidative damage in sensitive and tolerant. apple rootstocks (Malus sieversii and M. hupehensis). Although such stress inhibited seedling growth in both species, the extent of this effect differed greatly between the two genotypes. Under stress, dry weights were significantly lower from the hypoxia-sensitive M. sieversii than from the controls, but they increased significantly with the addition of AsA. The contents of hydrogen peroxide (H2O2) and superoxide radicals (O-2(center dot-)) were significantly increased in roots of both species exposed to hypoxia. This led to oxidative damage, which was indicated by the accumulation of malondialdehyde (MDA). However, that reaction in M. sieversii was significantly suppressed when AsA was supplemented. Exogenously applied AsA enhanced the activities of ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR), glutathione reductase (GR), superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in both species, albeit to a greater extent in M. sieversii than in M. hupehensis under hypoxia stress. Exogenously applied AsA raised its endogenous levels of both Malus species exposed to hypoxia, but to a greater extent in M. sieversii. AsA applications also slightly enhanced root glutathione (GSH) contents in stressed plants, but differences were not statistically significant between the treated and untreated plants. AsA possibly enhance hypoxia tolerance by elevating its endogenous level and improving the capacity of the ascorbate-glutathione cycle, thereby decreasing the occurrence of oxidative damage in the membranes. (C) 2013 Elsevier B.V. All rights reserved.