Doxorubicin Loaded Chitosan-W18O49 Hybrid Nanoparticles for Combined Photothermal-Chemotherapy
Doxorubicin Loaded Chitosan-W18O49 Hybrid Nanoparticles for Combined Photothermal-Chemotherapy
复制标题
用于光热化疗联合治疗的负载阿霉素的壳聚糖-W18O49 混合纳米颗粒
DOI：
10.1002/mabi.201700033
复制
发表时间：
2017-08-01
影响因子：
4.6
通讯作者：
Hu, Yong
中科院分区：
文献类型：
--
作者：
Yuan, Shanmei;Hua, Jisong;Hu, Yong
联合治疗比单一治疗对大多数形式的癌症更有效。本工作通过带正电的壳聚糖和带负电的W18 O 49纳米粒子之间的静电相互作用，制备了结合光热疗法和化疗的多柔比星壳聚糖-W18 O 49纳米粒子。通过动态光散射、透射电镜、细胞毒性、近红外荧光成像和肿瘤生长抑制实验研究了纳米粒子的体内外行为。这些纳米颗粒的平均尺寸约为110 nm，并显示出pH敏感的药物释放行为。在980 nm激光照射后，这些纳米颗粒显示出比单独的游离阿霉素或光热疗法更明显的对HeLa细胞的细胞毒性。体内实验证实，它们的抗肿瘤能力显著提高，导致在抑制肿瘤生长和延长小鼠寿命方面的上级效率。
Combined treatment is more effective than single treatment against most forms of cancer. In this work, doxorubicin loaded chitosan-W18O49 nanoparticles combined with the photothermal therapy and chemotherapy are fabricated through the electrostatic interaction between positively charged chitosan and negatively charged W18O49 nanoparticles. The in vitro and in vivo behaviors of these nanoparticles are examined by dynamic light scattering, transmission electron microscopy, cytotoxicity, near-infrared fluorescence imaging, and tumor growth inhibition experiment. These nanoparticles have a mean size around 110 nm and show a pH sensitive drug release behavior. After irradiation by the 980 nm laser, these nanoparticles show more pronounced cytotoxicity against HeLa cells than that of free doxorubicin or photothermal therapy alone. The in vivo experiments confirm that their antitumor ability is significantly improved, resulting in superior efficiency in impeding tumor growth and extension of the lifetime of mice.