Swimming in Aplysia brasiliana: behavioral and cellular effects of serotonin.
Swimming in Aplysia brasiliana: behavioral and cellular effects of serotonin.
复制标题
巴西海兔游泳：血清素的行为和细胞效应。
DOI：
10.1152/jn.1989.62.5.1163
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发表时间：
1989
影响因子：
2.5
通讯作者：
Pinsker,HM
中科院分区：
文献类型：
--
作者：
Parsons,DW;Pinsker,HM
1. 巴西海兔是一种海洋软体动物，通过双侧肉质副足反复异时拍动运动来游泳。患有双侧脑足结缔组织 (CPC) 损伤的动物在悬浮于基质上方时不会游泳，尽管强直性 CPC 刺激可以引起正常的足旁拍动。尽管在完整动物和解剖标本中，足旁开放期（POP）细胞（一组先前确定的神经元）会与足旁开放运动同步发射传出尖峰，但它们不太可能是初级足旁运动神经元。这些细胞在 CPC 刺激期间接收一个或多个大的、明显单突触的兴奋性突触后电位 (EPSP)，可有效产生游泳运动程序 (SMP)。 2. 在悬浮的 CPC 损伤动物中，注射产生平均血淋巴浓度为 10(-5) M 的血清素 (5-HT) 诱导全振幅旁足拍打。选定的拍动次数与正常悬浮游泳的频率相似。 3. 在悬吊的 CPC 损伤动物中，5-HT 注射引发了明显正常的游泳运动程序，该程序与足旁神经中大幅传出尖峰的同步爆发有关。在许多半完整的准备中，将食管环神经节暴露于 5-HT 会引发类似的节律性运动程序，但通常频率低于正常游泳或强直 CPC 刺激期间的频率。 4. 在离体神经节制备物中，5-HT 的浴应用产生了单个 POP 神经元的立即去极化和强直放电，随后在明显缺乏突触输入的情况下平滑且规则地爆发。在此类制剂中，由浴施加的 5-HT 引起的运动程序与由强直 CPC 刺激引起的运动程序不同，因为 5-HT 引起的节律性爆发通常在不同的 POP 神经元中不同步。在 5-HT 沐浴期间进行强效 CPC 刺激，可立即使 POP 神经元之间的爆发同步化。 5.在5-HT浴应用期间POP神经元的超极化（或去极化）增加（或减少）突发周期，但膜极化不改变强直CPC刺激期间引发的突发周期。
1. Aplysia brasiliana is a marine mollusk that swims by repeated metachronal flapping movements of its bilateral fleshy parapodia. Animals with bilateral cerebropedal connective (CPC) lesions do not swim when suspended above the substrate, although tonic CPC stimulation can elicit normal parapodial flapping. Although the parapodial opener-phase (POP) cells, a previously identified group of neurons, fire synchronous bursts of efferent spikes in-phase with parapodial opening movements in both intact animals and dissected preparations, they are not likely to be primary parapodial motoneurons. These cells receive one or more large, apparently monosynaptic excitatory postsynaptic potentials (EPSPs) during CPC stimulation that are effective in producing the swimming motor program (SMP). 2. In suspended CPC-lesioned animals, injections of serotonin (5-HT) that produce an average hemolymph concentration of 10(-5) M induced full-amplitude parapodial flapping. Selected episodes of flapping were similar in frequency to normal suspended swimming. 3. In suspended CPC-lesioned animals, 5-HT injections elicited an apparently normal swimming motor program that was associated with synchronous bursts of large-amplitude efferent spikes in the parapodial nerves. In many semi-intact preparations, exposing the circumoesophageal ganglia to 5-HT elicited a similar rhythmic motor program, but usually at a lower frequency than during normal swimming or during tonic CPC stimulation. 4. In isolated-ganglion preparations, bath application of 5-HT produced immediate depolarization and tonic firing of individual POP neurons, followed by smooth and regular bursting in the apparent absence of synaptic input. In such preparations, the motor program elicited by bath-applied 5-HT differed from the one elicited by tonic CPC stimulation in that the 5-HT-elicited rhythmic bursting usually was not synchronous in different POP neurons. Tonic CPC stimulation during bath applications of 5-HT produced immediate synchronization of bursts among the POP neurons. 5. Hyperpolarization (or depolarization) of a POP neuron during bath application of 5-HT increased (or decreased) the burst period, but membrane polarization did not change the burst period elicited during tonic CPC stimulation.