Proteome Analysis in a Mammalian Cell Line Reveals that PLK2 Is Involved in Avian Metapneumovirus Type C (aMPV/C)-Induced Apoptosis
Proteome Analysis in a Mammalian Cell Line Reveals that PLK2 Is Involved in Avian Metapneumovirus Type C (aMPV/C)-Induced Apoptosis
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哺乳动物细胞系的蛋白质组分析表明 PLK2 参与禽间质肺病毒 C 型 (aMPV/C) 诱导的细胞凋亡
DOI：
10.3390/v12040375
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发表时间：
2020-04-01
期刊：
影响因子：
4.7
通讯作者：
Liu，Jue
中科院分区：
文献类型：
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作者：
Quan，Rong;Wei，Li;Liu，Jue
禽偏肺病毒C亚型（aMPV/C）是一种严重危害我国养禽业的急性呼吸道疾病。在本研究中，我们首先使用iTRAQ定量蛋白质组学探索了aMPV/C感染的Vero细胞中的蛋白质谱。7034种蛋白质中共有921种被鉴定为被aMPV/C感染显著改变。通过Western印迹分析证实了三个选择的蛋白。生物信息学GO分析揭示了多种信号通路，包括细胞周期、胞吞作用、PI3K-Akt、mTOR、MAPK和p53信号通路，这些通路可能参与了病毒感染。在该分析中，我们发现PLK2表达被aMPV/C感染上调，并研究其是否有助于aMPV/C介导的细胞功能障碍。抑制PLK2减弱了aMPV/C诱导的活性氧（ROS）产生和p53依赖性凋亡，并减少了病毒释放。哺乳动物细胞系中的这些结果表明，PLK2高表达与aMPV/C诱导的细胞凋亡和病毒复制相关，为潜在的禽类宿主细胞对aMPV/C感染和抗病毒靶点的反应提供了新的见解。
Avian metapneumovirus subtype C (aMPV/C) causes an acute respiratory disease that has caused serious economic losses in the Chinese poultry industry. In the present study, we first explored the protein profile in aMPV/C-infected Vero cells using iTRAQ quantitative proteomics. A total of 921 of 7034 proteins were identified as significantly altered by aMPV/C infection. Three selected proteins were confirmed by Western blot analysis. Bioinformatics GO analysis revealed multiple signaling pathways involving cell cycle, endocytosis, and PI3K-Akt, mTOR, MAPK and p53 signaling pathways, which might participate in viral infection. In this analysis, we found that PLK2 expression was upregulated by aMPV/C infection and investigated whether it contributed to aMPV/C-mediated cellular dysfunction. Suppressing PLK2 attenuated aMPV/C-induced reactive oxygen species (ROS) production and p53-dependent apoptosis and reduced virus release. These results in a mammalian cell line suggest that high PLK2 expression correlates with aMPV/C-induced apoptosis and viral replication, providing new insight into the potential avian host cellular response to aMPV/C infection and antiviral targets.