Tuning of the molecular orientation of gel forming compounds and their effect on molecular shape selectivity in liquid chromatography
Tuning of the molecular orientation of gel forming compounds and their effect on molecular shape selectivity in liquid chromatography
复制标题
液相色谱中凝胶形成化合物分子取向的调节及其对分子形状选择性的影响
DOI：
10.1016/j.chroma.2013.11.032
复制
发表时间：
2014
影响因子：
4.1
通讯作者：
H. Ihara
中科院分区：
文献类型：
--
作者：
A. K. Mallik;S. Guragain;H. Hachisako;M. M. Rahman;M. Takafuji;H. Ihara
制备了双烷基化谷氨酸和氨基己二酸衍生的凝胶系统（G1 和 G2），然后将其接枝到二氧化硅颗粒上。然后将凝胶形成化合物接枝二氧化硅颗粒（Sil-G1 和 Sil-G2）用作固定相，用于多环芳烃（PAH）和生育酚的形状受限异构体的液相色谱分离。在固定到二氧化硅上之前和之后对 G1 和 G2 进行分析。元素分析和热重分析（TGA）结果表明，Sil-G1 和 Sil-G2 相中接枝了几乎相同数量的有机相。然而，色谱结果显示固定相之间的分子形状选择性存在很大差异。凝胶形成化合物的化学结构的微小变化可以改变分子取向以及液相色谱中分子识别的剧烈变化。官能团的分子取向影响形状受限异构体分离的相互作用机制。还描述了 Sil-G1 和 Sil-G2 相与其他商用色谱柱的形状选择性的比较。
Double-alkylated glutamic acid and aminoadipic acid derived gel systems (G1 and G2) have been prepared and then grafted onto silica particles. Gel-forming compounds-grafted silica particles (Sil-G1 and Sil-G2) were then applied for the liquid chromatographic separation of shape-constrained isomers of polycyclic aromatic hydrocarbons (PAHs) and tocopherols as stationary phases. G1 and G2 were analyzed before and after immobilization onto silica. Elemental analysis and thermogravimentric analysis (TGA) results showed almost the same amounts of organic phases were grafted in Sil-G1 and Sil-G2 phases. However, chromatographic results showed large differences of molecular-shape selectivity between the stationary phases. Little change in the chemical structures of the gel-forming compounds can change the molecular orientation as well as drastic change in molecular recognition in liquid chromatography. Molecular orientation of the functional groups affected the interaction mechanism for the separation of shape-constrained isomers. Comparison of the shape selectivity of Sil-G1 and Sil-G2 phases with other commercial columns is also described.