Activation peptide of coagulation factor IX improves the prognosis after traumatic brain injury
Activation peptide of coagulation factor IX improves the prognosis after traumatic brain injury
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凝血因子IX激活肽改善脑外伤后的预后
DOI：
10.1016/j.bbrc.2021.06.089
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Chiaki Hidai
中科院分区：
文献类型：
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作者：
Yuusuke Fujiwara ;Hisataka Kitano ;Takamitsu Yamamoto ;Shinichiro Kokubun ;Chiaki Hidai
最近，凝血因子IX及其活化肽被报道抑制血管内皮细胞的通透性。在这项研究中，合成的激活肽的治疗作用在创伤性脑损伤模型大鼠进行了研究。在脑挫伤中，血脑屏障功能障碍伴随血管通透性增加促进了损伤后神经病变的进展。模型大鼠采用控制性皮质撞击法。然后，每天给大鼠静脉注射350 μg/kg合成的活化肽或PBS作为对照，持续一个月。在一个月的观察期间进行了行为研究。对于形态学分析，进行宏观和显微镜观察。脑组织含水量用于评估水肿。采用伊文思蓝法检测血脑屏障功能。在神经系统检查和横梁行走中，治疗组大鼠的表现明显优于对照组大鼠。脑缺损体积的测量结果显示，治疗显著减少了82%。尼氏染色显示对照组大鼠撞击点附近神经细胞丢失，而治疗组大鼠撞击点附近神经细胞保留。治疗显著减少脑水肿和伊文思蓝的血管外渗漏。静脉注射合成的激活肽可显著减轻模型大鼠神经组织的损伤，改善神经功能。
Recently, coagulation factor IX and its activation peptide have been reported to suppress the permeability of vascular endothelial cells. In this study, the therapeutic effects of a synthesized activation peptide is investigated in traumatic brain injury model rats. In cerebral contusion, dysfunction of the blood brain barrier with increasing vascular permeability promotes the progression of neuropathy after injury. The model rats were generated by controlled cortical impact. Then, rats were intravenously injected with 350 μg/kg of the synthesized activation peptide or PBS as a control, every day for a month. Behavioral studies were conducted during a month of observation. For morphological analysis, macro- and microscopic observation were performed. Water content of brain tissue was used to assess edema. To assess the function of blood brain barrier, Evans Blue method was employed. In the neurological examinations and beam-walking, the treated rats performed significantly better than control rats. Measurements of cerebral defect volume showed that the treatment significantly reduced it by 82%. Nissl stain showed that neural cells adjacent to impacts were lost in control rats, but saved in treated rats. The treatment significantly reduced brain edema and extravascular leakage of Evans blue. Intravenous injection with a synthesized activation peptide significantly reduced damage to neural tissue and improved neural functioning in the model rats.