Analysis of sperm concentration and motility in a microfluidic device
Analysis of sperm concentration and motility in a microfluidic device
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DOI：
10.1007/s10404-010-0646-8
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发表时间：
2011-01-01
影响因子：
2.8
通讯作者：
Wo, Andrew M.
中科院分区：
文献类型：
--
作者：
Chen, Yu-An;Huang, Zi-Wei;Wo, Andrew M.
人们希望有一种能够快速、定量分析精子质量的家用设备，但尚未完全实现。为了帮助这方面的努力，本文提出了一种微流控装置能够量化精子质量的两个关键生育力相关的参数-活动精子浓度和活力。微装置产生供精子游动的流场，并且在指定时间内克服流动的精子在单独的通道中被推动沿着，并且通过电阻脉冲技术计数。并与临床常用的两种精子质量检测的对照方法-血细胞计数仪和精子质量分析仪进行比较。结果显示，精子传代产生的脉冲数与两种对照方法密切相关：0至335的脉冲数对应于0至19 x 10（6）/ml（血细胞计数器）的渐进活动精子浓度，精子活动指数对应于0至204（精子质量分析仪）。微型装置应适用于促进在家中自我评估精子质量。
A home-use device that allows rapid and quantitative sperm quality analysis is desirable but not yet fully realized. To aid this effort, this article presents a microfluidic device capable of quantifying sperm quality in terms of two critical fertility-related parameters-motile sperm concentration and motility. The microdevice produces flow field for sperms to swim against, and sperms that overcame the flow within a specified time are propelled along in a separate channel and counted via resistive pulse technique. Data are compared to two control methods clinically utilized for sperm quality exam-hemocytometer and the sperm quality analyzer. Results reveal the numbers of pulses generated by passage of sperms correlates strongly with the two control methods: pulse number from 0 to 335 corresponds to progressively motile sperm concentrations from 0 to 19 x 10(6)/ml (hemocytometer) and Sperm Motility Index from 0 to 204 (sperm quality analyzer). The microdevice should be applicable to facilitate self-assessment of sperm quality at home.