Thyrotropin cross-links to the thyrotropin receptor through both the alpha and beta subunits.
Thyrotropin cross-links to the thyrotropin receptor through both the alpha and beta subunits.
复制标题
促甲状腺素通过α和β亚基与促甲状腺素受体交联。
作者：
P. Buckland;C. Rickards;R. Howells;B. Smith
我们最近已经表明促甲状腺激素（TSH）的β亚基可以与TSH受体交联[巴克兰，Strickland，Pierce & Rees Smith（1985）Endocrinology（巴尔的摩）116，2122-2124;巴克兰，Strickland & Rees Smith（1985）Biochem.Soc.Trans.13，942-943]。然而，我们未能将α亚基与受体交联，使得该亚基在TSH-TSH-受体相互作用中的作用不确定。我们现在报告的成功交联的TSH α亚基的受体通过使用两种不同的交联试剂。因此，我们的研究表明，TSH的两个亚基形成激素受体结合位点的一部分。
We have recently shown that the beta subunit of thyrotropin (TSH) can be cross-linked to the TSH receptor [Buckland, Strickland, Pierce & Rees Smith (1985) Endocrinology (Baltimore) 116, 2122-2124; Buckland, Strickland & Rees Smith (1985) Biochem. Soc. Trans. 13, 942-943]. We failed, however, to cross-link the alpha subunit to the receptor, leaving the role of this subunit in the TSH-TSH-receptor interaction uncertain. We now report the successful cross-linking of the TSH alpha subunit to the receptor by the use of two different cross-linking reagents. Our studies suggest therefore that both subunits of TSH form part of the hormone's receptor-binding site.