Linking N2O emission from biochar-amended composting process to the abundance of denitrify (nirK and nosZ) bacteria community.
Linking N2O emission from biochar-amended composting process to the abundance of denitrify (nirK and nosZ) bacteria community.
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DOI：
10.1186/s13568-016-0208-x
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发表时间：
2016-12
期刊：
影响因子：
3.7
通讯作者：
Zou J
中科院分区：
文献类型：
--
作者：
Li S;Song L;Jin Y;Liu S;Shen Q;Zou J
粪便堆肥被认为是一个重要的人为一氧化二氮（N2O）的来源，有助于全球变暖。然而，生物炭对粪便堆肥N2O排放的影响很少被评估，特别是将其与丰富的分解细菌群落联系起来。这项研究的结果表明，生物炭修正案显着减少N2O的排放量从粪肥堆肥，主要是由于抑制nirK基因丰度的分解细菌。Pearson相关分析表明，nirK丰度与N2O通量呈显著正相关，nosZ密度与N2O通量呈显著负相关。同时，nirK基因丰度减去nosZ基因丰度与N2O通量之间也存在线性相关关系。此外，一个统计模型，用于估计N2O排放量的基础上的细菌分解功能基因的开发和验证，以充分适合所观察到的排放量。研究结果表明，添加生物质炭可以有效缓解堆肥过程中N2O的排放，而相关功能菌群的研究有助于了解堆肥过程中N2O的排放机理。
Manure composting has been recognized as an important anthropogenic source of nitrous oxide (N2O) contributing to global warming. However, biochar effect on N2O emissions from manure composting is rarely evaluated, especially by linking it to abundance of denitrifying bacteria community. Results of this study indicated that biochar amendment significantly reduced N2O emissions from manure composting, primarily due to suppression of the nirK gene abundance of denitrifying bacteria. Pearson’s correlation analysis showed a significant positive correlation between nirK abundance and N2O fluxes, while a negative correlation between nosZ density and N2O fluxes. Simultaneously, a linear correlation between nirK gene abundance minus nosZ gene abundance with N2O fluxes was also observed. In addition, a statistical model for estimating N2O emissions based on the bacterial denitrifying functional genes was developed and verified to adequately fit the observed emissions. Our results highlighted that biochar amendment would be an alternative strategy for mitigating N2O emissions during manure composting, and the information of related functional bacterial communities could be helpful for understanding the mechanism of N2O emissions.