Dual-electrode electrochemical detection for poly(dimethylsiloxane)-fabricated capillary electrophoresis microchips
Dual-electrode electrochemical detection for poly(dimethylsiloxane)-fabricated capillary electrophoresis microchips
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DOI：
10.1021/ac000160t
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发表时间：
2000-07-15
影响因子：
7.4
通讯作者：
Henry, CS
中科院分区：
文献类型：
--
作者：
Martin, RS;Gawron, AJ;Henry, CS
描述了采用双电极电化学检测的基于聚（二甲基硅氧烷）（基于PDMS）的微芯片电泳系统的开发。这是微芯片形式的双电极电化学检测和由 PDMS 制造的芯片上的电化学检测的第一份报告。本文描述的装置由包含分离和注射通道的 PDMS 顶层和沉积有金检测电极的底部玻璃层组成。这两层形成紧密的可逆密封，无需高温粘合， 可能会损害电极的稳定性。通道也可以暂时移除进行清洁，从而显着延长芯片的使用寿命。使用儿茶酚作为测试化合物来评估芯片的性能。响应在 10 至 500 μM 范围内呈线性，LOD (S/N 3) 为 4 μM，灵敏度为 45.9 pA/μM。在 200 至 400 V/cm 之间的保持强度下，儿茶酚的收集效率为 28.7% 至 25.9%。串联配置中的双电极检测被证明可用于选择性监测进行化学可逆氧化还原反应的物质以及未溶解混合物的电泳图中的峰识别。
The development of a poly(dimethylsiloxane)-based (PDMS-based) microchip electrophoresis system employing dual-electrode electrochemical detection is described. This is the first report of dual-electrode electrochemical detection in a microchip format and of electrochemical detection on chips fabricated from PDMS, The device described in this paper consists of a top layer of PDMS containing the separation and injection channels and a bottom glass layer onto which gold detection electrodes have been deposited, The two layers form a tight reversible seal, eliminating the need for high-temperature bonding, which can be detrimental to electrode stability. The channels can also be temporarily removed for cleaning, significantly extending the lifetime of the chip. The performance of the chip was evaluated using catechol as a test compound. The response was linear from 10 to 500 mu M with an LOD (S/N 3) of 4 mu M and a sensitivity of 45.9 pA/mu M. Collection efficiencies for catechol ranged from 28.7 to 25.9% at held strengths between 200 and 400 V/cm. Dual-electrode detection in the series configuration was shown to be useful for the selective monitoring of species undergoing chemically reversible redox reactions and for peak identification in the electropherogram of an unresolved mixture.