Use of PKA-mediated phenotypes for genetic and small-molecule screens in Schizosaccharomyces pombe
Use of PKA-mediated phenotypes for genetic and small-molecule screens in Schizosaccharomyces pombe
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DOI：
10.1042/bst20130159
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发表时间：
2013-12-01
影响因子：
3.9
通讯作者：
Hoffman, Charles S.
中科院分区：
文献类型：
--
作者：
de Medeiros, Ana Santos;Magee, Alexander;Hoffman, Charles S.
裂殖酵母裂殖酵母中的 PKA（蛋白激酶 A）控制参与新陈代谢、细胞生长和性发育的基因转录。在本综述中，我们讨论了与高或低 PKA 活性相关的表型，以及如何使用它们来进行影响 PKA 途径成分的遗传或小分子筛选。尽管我们最近的研究重点是异源表达的环核苷酸 PDE（磷酸二酯酶）的研究，但这些相同的方法可用于靶向其他粟酒裂殖酵母蛋白或其在 PKA 途径中起作用的功能等效的直系同源物。
PKA (protein kinase A) in the fission yeast Schizosaccharomyces pombe controls transcription of genes involved in metabolism, cell growth and sexual development. In the present review, we discuss phenotypes associated with either high or low PKA activity in the context of how they can be used to carry out genetic or small-molecule screens that affect components of the PKA pathway. Although our recent research has focused on the study of heterologously expressed cyclic nucleotide PDEs (phosphodiesterases), these same methods can be used to target other S. pombe proteins or their functionally equivalent orthologues that act in the PKA pathway.