Genetic Diversity of Plaque Mutans Streptococci with rep-PCR
Genetic Diversity of Plaque Mutans Streptococci with rep-PCR
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DOI：
10.1177/0022034510386375
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发表时间：
2011-03-01
影响因子：
7.6
通讯作者：
Childers, N. K.
中科院分区：
文献类型：
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作者：
Cheon, K.;Moser, S. A.;Childers, N. K.
变形链球菌（MS）是与龋齿病因学相关的关键生物体。使用通过过采样测试的概率，我们设计了这项研究，以确定评估基因型多样性所需的来自个体的MS分离株的最小数量。MS分离株的基因型重复基因外回文聚合酶链反应（rep-PCR）。对20株来自个体的分离株进行分析，儿童（N = 12）和成人（N = 10）的平均基因型分别为1.6和2.4。在一项后续研究中，将分离株数量减少至7-10株导致检测到多达4种基因型的理论概率高达78%。在35名儿童和10名成人中发现平均1.5种基因型。这些发现为MS基因分型研究的设计提供了证据，该研究可作为个体内基因型分析的模型。
Mutans streptococci (MS) are key organisms associated with the etiology of dental caries. Using probabilities that were tested by oversampling, we designed this study to determine the minimal number of MS isolates from an individual required to evaluate diversity of genotypes. MS isolates were genotyped by repetitive extragenic palindromic-polymerase chain-reaction (rep-PCR). Analysis of 20 isolates from individuals resulted in a mean of 1.6 and 2.4 genotypes in children (N = 12) and adults (N = 10), respectively. In a follow- up study, reducing the number of isolates to 7-10 resulted in a theoretical probability of up to 78% for detecting up to 4 genotypes. A mean of 1.5 genotypes was found in 35 children and 10 adults. These findings provide evidence for the design of studies of MS genotyping that can serve as a model for the analysis of genotypes within individuals.