CULTURE OF URETHRAL FIBROBLASTS - CELL MORPHOLOGY, PROLIFERATION AND EXTRACELLULAR-MATRIX SYNTHESIS
CULTURE OF URETHRAL FIBROBLASTS - CELL MORPHOLOGY, PROLIFERATION AND EXTRACELLULAR-MATRIX SYNTHESIS
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DOI：
10.1016/s0022-5347(17)35757-9
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发表时间：
1993-10-01
影响因子：
6.6
通讯作者：
HOWARD, PS
中科院分区：
文献类型：
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作者：
BASKIN, LS;MACARAK, EJ;HOWARD, PS
从胎牛尿道海绵体原代分离物中鉴定尿道成纤维细胞的体外形态和增殖活性。基于细胞的梭形形态和缺乏尿道平滑肌或上皮细胞的表型标志物特征的表达，将细胞鉴定为成纤维细胞。尿道成纤维细胞以稳定的速率增殖，并且在补充有10%新生小牛血清的培养基199的培养基中没有表现出生长的接触抑制。细胞外基质合成的免疫组织化学和代谢标记分析均表明存在I型和III型胶原蛋白以及纤连蛋白。细胞培养基中胶原成分的定量显示，尿道成纤维细胞合成85%的I型胶原，14%的III型胶原，
The in vitro morphology and proliferative activity of urethral fibroblasts were characterized from primary isolates of fetal bovine urethral spongiosum. Cells were identified as fibroblastic based on their spindle-shaped morphology and lack of expression of phenotypic markers characteristic of either urethral smooth muscle or epithelial cells. Urethral fibroblasts proliferated at a steady rate and did not exhibit contact-inhibition of growth in a culture medium of Medium 199 supplemented with 10% newborn calf serum. Both immunohistochemistry and metabolic labeling analyses of extracellular matrix synthesis demonstrated the presence of collagen types I and III, and fibronectin. Quantitation of the collagenous components of the cell medium revealed that urethral fibroblasts synthesize 85% type I collagen, 14% type III collagen and