Evaluation of the i-STAT portable point-of-care analyzer for determination of blood gases and acid-base status in newborn calves
Evaluation of the i-STAT portable point-of-care analyzer for determination of blood gases and acid-base status in newborn calves
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DOI：
10.1111/vec.12228
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发表时间：
2014-09-01
影响因子：
1.3
通讯作者：
Goetz, Eva
中科院分区：
文献类型：
--
作者：
Bleul, Ulrich;Goetz, Eva
目的-验证i-STAT，一种便携式手持式分析仪，可在食品动物实践中即时测量血气、酸碱、乳酸盐和其他血液变量，用于分析新生小牛的血样。设计-前瞻性观察研究。设置-大学教学医院。测量和主要结果-共分析了271份静脉血样的PvO（2），使用i-STAT和Rapidlab 248台式分析仪（作为参考方法）测定PvCO（2）、pH、碱过剩（BE）、HCO 3、静脉血氧饱和度（SvO（2））和总二氧化碳（TCO 2）。使用i-STAT和光度法测量L-乳酸。比较i-STAT和参考方法的结果。分析物BE、HCO 3和TCO 2在整个范围内显示出恒定的系统误差，其值分别比参考方法测得的值低2.3、1.9和2.0 mmol/L。基于2台分析仪的组合固有不精密度，并在校正系统误差的影响后，PvO（2）、HCO 3和SvO（2）在76%至91%的情况下均在可接受限度内。95%的PvCO（2）和BE的测量值在可接受的范围内。结论-i-STAT的总体性能良好，但BE、HCO 3和TCO 2除外，因此限制了其在临床研究中的实用性。然而，这种手持式设备允许快速、可靠和准确的即时血液分析，因此在牛的实践中是有用的。
Objective - To validate the i-STAT, a portable hand-held analyzer that allows point-of-care measurement of blood gases, acid-base, lactate, and other blood variables in food animal practice for analysis of blood samples of newborn calves.Design - Prospective observational study.Setting - University teaching hospital.Measurements and Main Results - A total of 271 venous blood samples were analyzed for PvO(2), PvCO(2), pH, base excess (BE), HCO3, venous saturation of oxygen (SvO(2)), and total carbon dioxide (TCO2) using an i-STAT and a Rapidlab 248 bench top analyzer that served as the reference method. L-lactate was measured using the i-STAT as well as photometrically. Results from the i-STAT and the reference methods were compared. The analytes BE, HCO3, and TCO2 showed a constant systematic error across the entire range with 2.3, 1.9, and 2.0 mmol/L lower values, respectively, than the values measured by the reference method. Based on the combined inherent imprecision of the 2 analyzers and after correcting the influences of systematic errors, the PvO(2), HCO3, and SvO(2) were within the acceptable limits in 76% to 91% of the cases. Ninety-five percent of the measurements of PvCO(2) and BE were within acceptable limits.Conclusions - The overall performance of the i-STAT was good except for BE, HCO3, and TCO2, thus limiting its usefulness in clinical studies. However this hand-held device allows rapid, reliable, and accurate point-of-care blood analyses and thus can be useful in bovine practice.