Recombinant interleukin 2 and gamma-interferon act synergistically on distinct steps of in vitro terminal human B cell maturation.
Recombinant interleukin 2 and gamma-interferon act synergistically on distinct steps of in vitro terminal human B cell maturation.
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重组白细胞介素 2 和 γ-干扰素在体外人 B 细胞终末成熟的不同步骤中发挥协同作用。
DOI：
10.1172/jci112418
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Fauci,AS
中科院分区：
文献类型：
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作者：
LethiBich-Thuy;Fauci,AS
研究了重组白细胞介素2 （IL-2）对未与金黄色葡萄球菌Cowan I或抗人IgM预孵育的人扁桃体B细胞体外分化的影响。结果表明，IL-2在2.5 ~ 2500 U IL-2/ml范围内可诱导含igg细胞的产生，并呈剂量依赖性。相反，在大多数实验中，培养上清中Ig的分泌量在25 U/ml时达到峰值。在IL-2刺激的培养中，可能存在能够合成Ig但不分泌其产生的Ig的细胞。在测试的许多因素中，我们发现γ -干扰素在单独使用时不会引发体外B细胞分化，但在IL-2刺激的培养物中，γ -干扰素可以诱导Ig分泌增加，而含Ig细胞的数量没有明显变化。讨论了γ -干扰素和IL-2在体外B细胞分化的后续步骤中起作用的可能性。
The effects of recombinant interleukin 2 (IL-2) on the in vitro differentiation of human tonsillar B cells which were not preincubated with Staphylococcus aureus Cowan I or with anti-human IgM were investigated. IL-2 was shown to induce the generation of Ig-containing cells in a dose-dependent fashion from 2.5 to 2,500 U IL-2/ml. Conversely, the quantities of Ig secreted in the culture supernatant were found in the majority of experiments to peak at 25 U/ml. The possible presence, in cultures stimulated with IL-2, of cells that were capable of synthesizing Ig but that did not secrete the Ig they have produced was investigated. Among a number of factors tested, we found that gamma-interferon, which did not trigger in vitro B cell differentiation when used alone, can induce an increased secretion of Ig without noticeable change in the number of Ig-containing cells in cultures stimulated with IL-2. The possibility that gamma-interferon and IL-2 act on subsequent steps of in vitro B cell differentiation is discussed.