Evaluation of Th1 and Th2 immune response in clinical and sub-clinical scrub typhus infection
Evaluation of Th1 and Th2 immune response in clinical and sub-clinical scrub typhus infection
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DOI：
10.1016/j.humimm.2019.03.013
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发表时间：
2019-07-01
期刊：
影响因子：
2.7
通讯作者：
Khan, Siraj Ahmed
中科院分区：
文献类型：
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作者：
Bora, Trishna;Khan, Siraj Ahmed
由革兰氏阴性细胞内细菌-恙虫病东方体引起的恙虫病（ST）是包括印度在内的东南亚地区发热性疾病的主要原因之一。临床表现可以从无症状到严重致命不等。Th细胞介导的免疫在动物模型中对ST感染起重要作用。然而，关于保护性免疫的人类数据有限。进行本研究以鉴定可以在保持临床无症状/亚临床的个体中赋予保护的宿主免疫相关性。在临床和亚临床感染中研究了血清细胞因子谱和Th 1（TNF-α、IFN-γ、IL-2）和Th 2（IL-10、IL-6、IL-4）细胞因子的mRNA表达水平。观察到Th 1/Th 2模式不涉及人ST感染，无论是有症状的还是无症状的表现。而IL-10血清水平和基因表达水平则有显著差异。这项研究表明IL-10在疾病表型呈现中可能发挥作用。IL-10的过度产生被认为是导致疾病严重程度的重要因素，而ST感染中低水平的IL-10可能存在保护性免疫机制。
Scrub typhus (ST), caused by a gram negative intracellular bacteria- Orientia tsutsugamushi, is one among the leading causes of febrile illness across Southeast Asia, including India. Clinical presentation can vary from asymptomatic to severely fatal. Thl-cell mediated immunity has been suggested to play an important role against ST infection in animal models. However, human data on protective immunity are limited. The present study was undertaken to identify host immune correlates that could confer protection in individuals that remain clinically asymptomatic/sub-clinical. Serum cytokine profiling and mRNA expression levels of Th1 (TNF-alpha, IFN-gamma, IL-2) and Th2 (IL-10, IL-6, IL-4) cytokines was studied amongst the clinical and sub-clinical infections. It was observed that a Th1/Th2 pattern is not involved in human ST infection irrespective of being a symptomatic or asymptomatic presentation. However, significant difference was observed in IL-10 serum and gene expression levels. This study suggests a possible role of IL-10 in disease phenotypic presentation. Over-production of IL-10 was found to be a significant factor contributing to the severity of the disease whereas a protective immune mechanism might exist with a low level of IL-10 in ST infection.