Cell shape changes indicate a role for extrinsic tensile forces in Drosophila germ-band extension
Cell shape changes indicate a role for extrinsic tensile forces in Drosophila germ-band extension
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DOI：
10.1038/ncb1894
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发表时间：
2009-07-01
影响因子：
21.3
通讯作者：
Sanson, Benedicte
中科院分区：
文献类型：
--
作者：
Butler, Lucy C.;Blanchard, Guy B.;Sanson, Benedicte
果蝇生殖带延伸（GBE）是一个例子的收敛和延伸运动，延长和缩小胚胎组织。为了了解组织形态发生背后的集体细胞行为，我们在GBE期间连续量化细胞嵌入和细胞形状变化。我们发现，快速，早期阶段的GBE取决于细胞形状的变化，除了细胞嵌入。在前后模式突变体中，例如差距基因Kruppel的突变体，有缺陷的极化细胞嵌入通过前后细胞伸长的增加来补偿，使得延伸的初始速率保持不变。细胞行为的时空模式表明，前后张力使胚带变形，导致细胞被动地改变形状。扭转突变体胚胎的前后细胞伸长率降低，缺乏中胚层。我们提出，细胞形状的变化，生殖带的延伸是一个被动的反应，机械力所造成的内陷中胚层。
Drosophila germ-band extension (GBE) is an example of the convergence and extension movements that elongate and narrow embryonic tissues. To understand the collective cell behaviours underlying tissue morphogenesis, we have continuously quantified cell intercalation and cell shape change during GBE. We show that the fast, early phase of GBE depends on cell shape change in addition to cell intercalation. In antero-posterior patterning mutants such as those for the gap gene Kruppel, defective polarized cell intercalation is compensated for by an increase in antero-posterior cell elongation, such that the initial rate of extension remains the same. Spatio-temporal patterns of cell behaviours indicate that an antero-posterior tensile force deforms the germ band, causing the cells to change shape passively. The rate of anteroposterior cell elongation is reduced in twist mutant embryos, which lack mesoderm. We propose that cell shape change contributing to germ-band extension is a passive response to mechanical forces caused by the invaginating mesoderm.