Virulence in mice of Orientia tsutsugamushi isolated from patients in a new endemic area in Japan
Virulence in mice of Orientia tsutsugamushi isolated from patients in a new endemic area in Japan
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DOI：
10.1111/j.1348-0421.1996.tb01135.x
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发表时间：
1996-01-01
影响因子：
2.6
通讯作者：
Yamashita, T
中科院分区：
文献类型：
--
作者：
Nagano, I;Kasuya, S;Yamashita, T
本文报道了从日本岐阜县恙虫病流行区分离的4株恙虫病东方体（KN-1、KN-2、KN-3和GJ-1）的毒力。其中，KN-1（鉴定为川崎型）、GJ-1（鉴定为Kuroki型）和KN-2菌株对BALB/c小鼠和CH 3/HeJ小鼠均无致死性，即使在高剂量（10（6）x为50%小鼠感染剂量）下也是如此。另一方面，发现KN-3菌株的毒性足以杀死BALB/c小鼠。在原型菌株（Gilliam，Karp和Kato）中，Karp和Kato菌株对小鼠表现出高毒力，而Gilliam菌株仅杀死敏感小鼠品系。感染KN-1和KN-2菌株的BALB/c小鼠在感染的第一周表现出显著的脾肿大和中度腹水积聚，而这些症状在感染KN-3、Karp和Kato菌株的第二周变得突出。感染GJ-1菌株后，未观察到这些症状。强毒株感染诱导的抗体应答低于中、弱毒株。
Four strains of Orientia tsutsugamushi (KN-1, KN-2, KN-3 and GJ-1) isolated from patients in an area of Gifu Prefecture, Japan, in which tsutsugamushi disease is newly endemic, were examined for their virulence in mice. Among these, KN-1 (identified as Kawasaki type), GJ-1 (identified as Kuroki type) and KN-2 strains were found to be non-lethal for BALB/c mice as well as CH3/HeJ mice, even with high doses (10(6) x being the 50% mouse infectious dose). On the other hand, the KN-3 strain was found to be sufficiently virulent to kill BALB/c mice. Among the prototype strains (Gilliam, Karp and Kato), the Karp and Kato strains exhibited high virulence to mice, while the Gilliam strain killed only a susceptible strain of mouse. BALB/c mice infected with KN-1 and KN-2 strains showed significant splenomegaly and moderate ascites accumulation in the first week of infection, while these symptoms became prominent during the second week of infection using KN-3, Karp and Kato strains. After infection with the GJ-1 strain, these symptoms were not observed. Antibody responses induced by infections with highly virulent strains were lower than that with low or intermediate virulent strains.