Peripheral blood CD4+T cell populations by CD25 and Foxp3 expression as a potential biomarker: reflecting inflammatory activity in chronic obstructive pulmonary disease
Peripheral blood CD4+T cell populations by CD25 and Foxp3 expression as a potential biomarker: reflecting inflammatory activity in chronic obstructive pulmonary disease
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外周血 CD4 T 细胞群 CD25 和 Foxp3 表达作为潜在的生物标志物：反映慢性阻塞性肺疾病的炎症活动
DOI：
10.2147/copd.s208977
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发表时间：
2019-01-01
影响因子：
2.8
通讯作者：
Xiong, Xian-Zhi
中科院分区：
文献类型：
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作者：
Meng, Zhao-Ji;Wu, Jiang-Hua;Xiong, Xian-Zhi
背景：CD4+ T 细胞中 CD25 和 Foxp3 表达的时间动态变化是由 T 细胞受体 (TCR) 信号强度或频率引发的。目前缺乏评估COPD活动性的外周标志物，本研究旨在探讨基于CD25和Foxp3表达的外周CD4+ T细胞群是否可以作为COPD炎症活动性的指标。方法：采用流式细胞术测定不同人群外周血CD4+CD25+Foxp3+ T细胞的分布和表型特征。体外探索CD25和Foxp3表达分化CD4+ T细胞群的模型。结果：AECOPD患者外周血CD4+CD25+Foxp3- T细胞和CD4+CD25+Foxp3+ T细胞的频率升高，而未经全身治疗的SCOPD患者外周血CD4+CD25-Foxp3+ T细胞的频率升高。表型分析显示CD4+CD25+Foxp3-T细胞、CD4+CD25+Foxp3+T细胞和CD4+CD25-Foxp3+T细胞接受了抗原刺激并且类似于中枢记忆或效应记忆T细胞。 CD4+ T 细胞群通过 CD25 和 Foxp3 表达的分化是由 TCR 信号决定的。配对研究表明，从 AECOPD 到恢复期，同一患者中 CD4+CD25+Foxp3- T 细胞、CD4+CD25+Foxp3+ T 细胞和 CD4+CD25-Foxp3+ T 细胞的频率下降，而 CD4+CD25-Foxp3- T 细胞的频率增加。结论：总的来说，我们提出 CD25 和 Foxp3 对 CD4+ T 细胞群的动态变化表达可以作为反映 COPD 炎症活动的潜在生物标志物。
Background: The temporally dynamic changes of CD25 and Foxp3 expression in CD4+ T cells are initiated by T cell receptor (TCR) signals strength or frequency. There is a deficiency of peripheral markers for assessing COPD activity, and the current study was conducted to explore whether peripheral CD4+ T cell populations based on CD25 and Foxp3 expression could serve as an indicator for COPD inflammatory activity.Methods: The distribution and phenotypic characteristics of CD4+CD25+Foxp3+ T cells from peripheral blood in different populations were determined by flow cytometry. The model for the differentiation of CD4+ T cells populations by CD25 and Foxp3 expression was explored in vitro.Results: The frequencies of peripheral CD4+CD25+Foxp3- T cells and CD4+CD25+Foxp3+ T cells were increased in AECOPD patients, whereas the frequency of CD4+CD25-Foxp3+ T cells was increased in SCOPD patients without receiving systemic treatment. Phenotypic analysis revealed that CD4+CD25+Foxp3- T cells, CD4+CD25+Foxp3+ T cells and CD4+CD25-Foxp3+ T cells had received antigenic stimulation and resembled central memory or effector memory T cells. The differentiation of CD4+ T cells populations by CD25 and Foxp3 expression was dictated by TCR signals. The paired study indicated that the frequencies of CD4+CD25+Foxp3- T cells, CD4+CD25+Foxp3+ T cells and CD4+CD25-Foxp3+ T cells were decreased while the frequency of CD4+CD25-Foxp3- T cells were increased in the same patients from AECOPD to convalescence.Conclusions: Collectively, we propose that the dynamic changes of CD4+ T cell populations by CD25 and Foxp3 expression could function as potential biomarkers for reflecting inflammatory activity in COPD.