Dopamine 3-sulfate inhibits aldosterone secretion in cultured bovine adrenal cells.
Dopamine 3-sulfate inhibits aldosterone secretion in cultured bovine adrenal cells.
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Dopamine 3-sulfate 可抑制培养的牛肾上腺细胞中醛固酮的分泌。
DOI：
10.1152/ajpendo.1984.247.4.e431
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
A. De Léan
中科院分区：
文献类型：
--
作者：
K. Rácz;N. Buu;O. Kuchel;A. De Léan
体外实验表明，多巴胺能抑制牛肾上腺细胞分泌醛固酮（Aldo）。由于牛血浆中总循环多巴胺（DA）的大部分（72%）以磺胺偶联形式存在（6.8 +/- 3.4 pmol/ml），并且在牛肾上腺皮质中可以发现游离DA、硫酸DA和酚硫转移酶（PST）活性，因此我们研究了游离DA对血管紧张素II- (ANG II)刺激的Aldo分泌的抑制作用是否也可以被磺胺偶联DA类似物复制。测试前培养3天的牛肾上腺细胞对10(-8)M ANG II刺激的反应是Aldo输出增加10- 12倍。游离DA和硫酸DA 3的加入部分抑制了这种刺激作用，而硫酸DA 4无活性。游离DA和硫酸DA - 3在100 μ m浓度下均产生最大的抑制作用，Aldo的分泌量分别减少61%（游离DA）和44%（硫酸DA - 3）。DA和DA 3-硫酸盐对Aldo分泌的抑制曲线略有不同，这不能用水解过程中游离DA的释放来解释，因为在与硫代共轭DA孵育后，细胞中检测到的游离DA不到1%。这些体外实验结果表明，硫酸DA 3可能在肾上腺水平上有效地补充多巴胺能抑制Aldo分泌。
It has been demonstrated that dopamine inhibits the aldosterone (Aldo) secretion in bovine adrenal cells in vitro. Because the majority of the total circulating dopamine (DA) in bovine plasma (72%) is present in sulfoconjugated form (6.8 +/- 3.4 pmol/ml) and free DA, DA sulfate, and phenolsulfotransferase (PST) activity can be found in the bovine adrenal cortex, we examined whether the inhibitory action of free DA on angiotensin II- (ANG II) stimulated Aldo secretion by cultured bovine adrenal cells can also be reproduced by sulfoconjugated DA analogues. Cells from the bovine adrenal glands cultured for 3 days before testing responded to 10(-8) M ANG II stimulation by a 10- to 12-fold increase in Aldo output. This stimulatory effect was partially inhibited by the addition of free DA and DA 3-sulfate, whereas DA 4-sulfate was inactive. Both free DA and DA 3-sulfate produced their maximal inhibitory effect at a concentration of 100 microM, with a decrease in Aldo secretion by 61% (free DA) and 44% (DA 3-sulfate). The slightly different inhibition curves for DA and for DA 3-sulfate on Aldo secretion cannot be explained by liberation of free DA through hydrolytic processes because less than 1% free DA was detected after the incubation of the cells with sulfoconjugated DA. These in vitro findings suggest that DA 3-sulfate may be effective in complementing the dopaminergic inhibition of Aldo secretion at the adrenal level.