Colorimetric determination of glutathione by using a nanohybrid composed of manganese dioxide and carbon dots
Colorimetric determination of glutathione by using a nanohybrid composed of manganese dioxide and carbon dots
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DOI：
10.1007/s00604-018-2830-6
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发表时间：
2018-05
影响因子：
5.7
通讯作者：
Qian Wang;H. Pang;Yongqiang Dong;Yuwu Chi;Fengfu Fu
中科院分区：
文献类型：
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作者：
Qian Wang;H. Pang;Yongqiang Dong;Yuwu Chi;Fengfu Fu
将一种富含羧基的单层碳基点(CDS)与二氧化锰(MnO2)进行杂化。得到的纳米杂化是稳定的，可以很好地分散在水中。MnO2能够氧化底物3，3‘，5，5’-四甲基联苯胺(TMB)生成蓝色产物，在谷胱甘肽(GSH)存在下，其吸收峰(655 Nm)减弱。建立了一种灵敏、选择性的谷胱甘肽比色分析方法，在10~0.1mGSH浓度范围内具有良好的线性关系，检出限为0.095 M。将该方法应用于添加胎牛血清中谷胱甘肽的测定，获得了良好的回收率。图形摘要二氧化锰/碳基点纳米杂化材料(MnO2/CDS)的制备图解。可用于3，3‘，5，5’-四甲基联苯胺(TMB)变色比色测定谷胱甘肽(GSH)。
A kind of single-layer carbon based dots (CDs) with abundant carboxyl functional groups was hybridized with manganese dioxide (MnO2). The resulting nanohybrid is stable and can be well dispersed in water. MnO2is capable of oxidizing the substrate 3,3′5,5′-tetramethylbenzidine (TMB) to form a blue product whose absorption (peaking at 655 nm) fades in the presence of glutathione (GSH). A sensitive and selective colorimetric GSH assay was worked out that has a linear response in the 10 to 0.1 µM GSH concentration range, with a 0.095 μM detection limit. The method was applied to the determination of GSH in spiked fetal calf serum where it gave excellent recoveries.Graphical abstractSchematic of the preparation of a nanohybrid composed of manganese dioxide and carbon based dots (MnO2/CDs). They can be used for the colorimetric detection of glutathione (GSH) based on the color change of 3,3′5,5′-tetramethylbenzidine (TMB).