Overexpression of zeaxanthin epoxidase gene enhances the sensitivity of tomato PSII photoinhibition to high light and chilling stress.
Overexpression of zeaxanthin epoxidase gene enhances the sensitivity of tomato PSII photoinhibition to high light and chilling stress.
复制标题
DOI：
10.1111/j.1399-3054.2007.01016.x
复制
发表时间：
2008-03
影响因子：
6.4
通讯作者：
Ning Wang;W. Fang;Han Han-Han;N. Sui;Bin Li;Q. Meng
中科院分区：
文献类型：
--
作者：
Ning Wang;W. Fang;Han Han-Han;N. Sui;Bin Li;Q. Meng
番茄(Lycpers siconesculentum Mill.)分离到玉米黄质环氧酶基因(LeZE)。推导的氨基酸序列与其他植物中的玉米黄质环氧酶有很高的同源性。Northern印迹分析表明，野生型(WT)中LeZE基因的积累不受光照和温度的诱导，而是受昼夜节律的调节。在花椰菜花叶病毒35S启动子(35S-CaMV)的控制下获得了正义转基因植株。Northern和Western印迹分析证实正义LeZE基因已转入番茄基因组并高效表达。转基因植株的(A+Z)/(V+A+Z)/(V+A+Z)比值和非光化学猝灭值均低于WT植株。在两种胁迫条件下，转基因植株的O(2)释放速率和PSII的最大光化学效率(Fv/Fm)比对照植株下降得更快，恢复得更慢。这些结果表明，在强光和低温胁迫下，LeZE的过度表达削弱了番茄叶黄素循环的功能，加剧了PSII光抑制。
A tomato (Lycopersicon esculentum Mill.) zeaxanthin epoxidase gene (LeZE) was isolated. The deduced amino acid sequence of LeZE showed high identities with zeaxanthin epoxidase in other plant species. Northern blot analysis showed that the mRNA accumulation of LeZE in the wild-type (WT) was not induced by light and temperature but regulated by the diurnal rhythm. The sense transgenic plants were obtained under the control of the cauliflower mosaic virus 35S promoter (35S-CaMV). Northern and western blot analysis confirmed that sense LeZE was transferred into the tomato genome and overexpressed. The ratio of (A + Z)/(V + A + Z) and the values of non-photochemical quenching were lower in transgenic plants than in WT plants under high light and chilling stress with low irradiance. The O(2) evolution rate and the maximal photochemical efficiency of PSII (Fv/Fm) in transgenic plants decreased more quickly during both stresses and recovered slower than that in WT under optimal conditions. These results suggested that overexpression of LeZE impaired the function of the xanthophyll cycle and aggravated PSII photoinhibition in tomato under high light and chilling stress.