RNA and Protein Synthesis in the Response of Pigeon Crop-Sac to Prolactin.∗
RNA and Protein Synthesis in the Response of Pigeon Crop-Sac to Prolactin.∗
复制标题
鸽子嗉囊对催乳素反应中的 RNA 和蛋白质合成。*
DOI：
10.3181/00379727-126-32580
复制
发表时间：
1967
影响因子：
--
通讯作者：
C. S. Nicoll
中科院分区：
文献类型：
--
作者：
W. Sherry;C. S. Nicoll
总结皮内注射放线菌素D或嘌呤霉素局部超过一侧的鸽子作物囊抑制组织的反应，全身给予催乳素。从催乳素刺激的作物囊粘膜组织中提取的RNA在作物囊上皮内注射时具有高度的作物囊刺激活性。相比之下，从鸽子肝组织或从在提取前加入催乳素的肝脏中提取的类似RNA组分没有这种活性。RNase对作物囊RNA的水解完全消除了其对作物囊的刺激能力。这些结果表明，RNA和蛋白质的合成是必要的作物囊的催乳素反应的表达。
Summary Intradermal injection of either actinomycin D or puromycin locally over 1 side of the pigeon crop-sac inhibited the tissue's response to systemically administered prolactin. RNA extracted from prolactin-stimulated crop-sac mucosal tissue had a high degree of crop-sac stimulating activity when injected intradermally over the crop-sac. In contrast, similar RNA fractions extracted from pigeon liver tissue or from liver to which prolactin was added prior to extraction were devoid of this activity. Hydrolysis of the crop-sac RNA by RNase completely eliminated its crop-sac stimulating capacity. These results indicate that RNA and protein synthesis is prerequisite for the expression of the crop-sac response to prolactin.