Western equine encephalomyelitis virus infection affects the life table characteristics of Culex tarsalis (Diptera: Culicidae)
Western equine encephalomyelitis virus infection affects the life table characteristics of Culex tarsalis (Diptera: Culicidae)
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DOI：
10.1603/0022-2585-41.5.982
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发表时间：
2004-09-01
影响因子：
2.1
通讯作者：
Fang, Y
中科院分区：
文献类型：
--
作者：
Mahmood, F;Reisen, WK;Fang, Y
以每毫升肝素化鸡血液中含4和6个log（10）空斑形成单位（PFU）的西方马脑脊髓炎病毒（WEEV）为感染源，对实验感染的跗跖库蚊（Culex tarsalis Coquillett）雌蚊的生命表特征进行了比较。雌性连续提供10%的蔗糖和产卵基质，每天通过生物膜喂食器血餐。每天监测死亡率（死亡雌性）和生殖力（每只雌性的雌性卵），直至所有雌性死亡。总体而言，当通过空斑试验检测时，两个病毒感染组中的198名女性中有94%在死亡时为WEEV阳性;死亡时的平均身体病毒滴度在组间没有差异。WEEV感染显著改变了Cx的生命表特征。跗骨感染队列的感染预期寿命（以天计）（e（x））、每代每只雌性的雌性卵的生殖努力（R-o）和世代时间（T）（以天计）显著低于未感染对照组，而感染队列的每只雌性每天的雌性卵的生殖率（r（c））高于未感染队列。与WEEV感染数据（显示相似的身体滴度）一致，4和6 log（10）PFU处理组的生命表参数之间几乎没有差异。在第17-40天之间观察到存活率的最大差异，此时感染的垂死雌性中的病毒滴度最大。我们的数据扩展了最近的研究，表明蚊子感染脑炎病毒会降低预期寿命和健康。
The life table attributes of Culex tarsalis Coquillett females infected experimentally by feeding on 4 and 6 log(10) plaque-forming units (PFU) of western equine encephalomyelitis virus (WEEV) per milliliter of heparinized chicken blood were compared with an uninfected control group. Females continually were offered 10% sucrose and an oviposition substrate and daily a blood meal through a biomembrane feeder. Mortality (dead females) and fecundity (female eggs per female) were monitored daily until all females died. Overall, 94% of 198 females in the two virus-infected groups were positive for WEEV at death when tested by plaque assay; the average body virus titer at death did not differ between groups. WEEV infection significantly altered the life table characteristics of Cx. tarsalis. Life expectancy at infection in days (e(x)), reproductive effort in female eggs per female per generation (R-o), and generation time (T) in days for the infected cohorts were significantly lower than for the uninfected controls, whereas the reproductive rate (r(c)) in female eggs per female per day was higher for infected than uninfected cohorts. In agreement with the WEEV infection data that showed similar body titers, there were few differences between the life table parameters for the 4 and 6 log(10) PFU treatment groups. Greatest differences were observed for survivorship between days 17-40 when virus titers in infected dying females were greatest. Our data extend recent studies that indicate mosquito infection with encephalitis viruses has a cost of reduced life expectancy and fitness.