Rotavirus subunit vaccines.
Rotavirus subunit vaccines.
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轮状病毒亚单位疫苗。
DOI：
10.1007/978-3-7091-6553-9_21
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Estes,MK
中科院分区：
文献类型：
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作者：
Conner,ME;Crawford,SE;Barone,C;O'Neal,C;Zhou,YJ;Fernandez,F;Parwani,A;Saif,LJ;Cohen,J;Estes,MK
我们评估了用于人类和动物的轮状病毒亚单位疫苗。昆虫细胞与表达人、牛或猿轮状病毒 VP2、VP4、VP6 或 VP7 的单个杆状病毒重组体的组合共感染，以产生病毒样颗粒 (VLP)。为了确定VLPs免疫是否可以诱导主动保护性免疫，对兔子进行胃肠外注射VLPs，并评估兔ALA轮状病毒攻击的免疫反应和保护作用。获得了完全或部分保护，表明用VLP进行肠外免疫可诱导主动保护性免疫。我们还研究了是否可以用有限数量的广泛反应性 VP7 蛋白或嵌合颗粒（单个颗粒上的多种 VP7 类型）诱导异型免疫反应。通过用含有 G3 VP7 或 G1 VP7 和嵌合 G1/G3 VLP 的 VLP 免疫小鼠来确定该方法的可行性。 G1 VLP 诱导产生广泛反应性的中和抗体。 VLP 还已成功用于增强奶牛的泌乳（初乳和牛奶）免疫力。综上所述，这些结果表明，当胃肠外给药时，VLP可以成为兔、小鼠和奶牛的有效免疫原，有限数量的VLP可能足以产生广泛保护性的疫苗，并且G3 VLP可以作为用于牛的有效亚单位疫苗。
We evaluated rotavirus subunit vaccines for use in humans and animals. Insect cells were co-infected with combinations of individual baculovirus recombinants expressing human, bovine or simian rotavirus VP2, VP4, VP6 or VP7 to produce virus-like particles (VLPs). To determine whether immunization with VLPs could induce active protective immunity, VLPs were administered parenterally to rabbits, and the immune response and protection from rabbit ALA rotavirus challenge were evaluated. Complete or partial protection was attained, showing that parenteral immunization with VLPs induces active protective immunity. We also examined whether heterotypic immune responses could be induced with a limited number of broadly reactive VP7 proteins or with chimeric particles (multiple VP7 types on individual particles). The feasibility of this approach was determined by immunizing mice with VLPs containing a G3 VP7 or G1 VP7 and chimeric G1/G3 VLPs. Broadly reactive neutralizing antibody was induced by the G1 VLPs. VLPs also have been successfully used to boost lactogenic (colostral and milk) immunity in dairy cows. Taken together, these results show that VLPs can be effective immunogens in rabbits, mice and dairy cattle when administered parenterally, a limited number of VLPs may be sufficient to produce a broadly protective vaccine, and G3 VLPs may serve as an effective subunit vaccine for use in bovines.