Localization of the glucocorticoid receptor in testis and accessory sexual organs of male rat
Localization of the glucocorticoid receptor in testis and accessory sexual organs of male rat
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DOI：
10.1016/0303-7207(93)90036-j
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发表时间：
1993-09
影响因子：
4.1
通讯作者：
R. Schultz;J. Isola;M. Parvinen;J. Honkaniemi;A. Wikström;J. Gustafsson;M. Pelto-huikko
中科院分区：
文献类型：
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作者：
R. Schultz;J. Isola;M. Parvinen;J. Honkaniemi;A. Wikström;J. Gustafsson;M. Pelto-huikko
应用光镜和电镜免疫细胞化学方法，研究了成年大鼠睾丸、附睾、射精管、精囊和前列腺中糖皮质激素受体样免疫反应（GR-LI）的定位。在睾丸组织中，GR-LI可见于间质细胞、巨噬细胞、成纤维细胞、平滑肌细胞和血管内皮细胞的细胞核内。此外，GR-LI在精子发生周期的XIII-XIV和I-III期的偶线期和粗线期早期的一些曲细精管初级精母细胞中观察到。其他生精细胞和支持细胞无染色。在附睾、输精管和前列腺的管周肌样细胞、成纤维细胞和基底细胞中也发现GR-LI。GR-LI在初级精母细胞和Leydig细胞中的定位表明，糖皮质激素直接影响睾丸中精子和类固醇的生成。GR-LI的男性附属性器官的功能，基质细胞的情况下，表明他们不是糖皮质激素的目标。
Localization of glucocorticoid receptor-like immunoreactivity (GR-LI) was studied in adult rat testis, epididymis, ejaculatory duct, seminal vesicle and prostate by light and electron microscopic immunocytochemistry. In the interstitium of the testis GR-LI was seen in the nuclei of Leydig cells, macrophages, fibroblasts, smooth muscle cells and endothelial cells of blood vessels. Furthermore, GR-LI was observed in zygotene and early pachytene primary spermatocytes of some seminiferous tubules during stages XIII–XIV and I–III of the spermatogenic cycle. Other spermatogenic cells and Sertoli cells were devoid of staining. GR-LI was also found in peritubular myoid cells, fibroblasts and basal cells of the epididymis, vas deferens and prostate. Localization of GR-LI in primary spermatocytes and Leydig cells suggests that glucocorticoids directly affect spermato- and Steroidogenesis in the testis. The absence of GR-LI from functional, stromal cells of the male accessory sexual organs suggests that they are not targets for glucocorticoid hormones.