Ultrasensitive and Label-Free Detection of the Measles Virus Using an N-Heterocyclic Carbene-Based Electrochemical Biosensor
Ultrasensitive and Label-Free Detection of the Measles Virus Using an N-Heterocyclic Carbene-Based Electrochemical Biosensor
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DOI：
10.1021/acssensors.0c01250
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发表时间：
2020-09-25
期刊：
影响因子：
8.9
通讯作者：
Birss, Viola, I
中科院分区：
文献类型：
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作者：
Mayall, Robert M.;Smith, Christene A.;Birss, Viola, I
鉴于当前对快速准确检测 COVID-19 病毒的强烈需求，我们报告了一种非常适合此目的的平台技术，并使用完整的麻疹病毒进行演示。麻疹病毒感染病例正在迅速增加，但目前用于监测病毒的诊断工具依赖于缓慢（>1 小时）的技术。在这里，我们展示了第一个能够在几分钟内检测麻疹病毒且无需预处理步骤的生物传感器。关键的传感元件是一个电极，该电极涂有包含麻疹抗体的自组装单层，并通过 N-杂环卡宾 (NHC) 固定。通过电阻变化检测完整病毒，对 10-100 μg/mL 完整麻疹病毒给出线性响应，无需标记或处理样品。检测限为6μg/mL，处于可引起灵长类动物感染的浓度下限。基于 NHC 的生物传感器被证明优于基于硫醇的系统，产生大约 10 倍的响应，并且在重复测量和长期存储方面具有显着更高的稳定性。因此，这种基于 NHC 的生物传感器代表了麻疹病毒快速检测以及作为检测其他感兴趣的生物靶标的平台技术的重要发展。
With the current intense need for rapid and accurate detection of viruses due to COVID-19, we report on a platform technology that is well suited for this purpose, using intact measles virus fora demonstration. Cases of infection due to the measles virus are rapidly increasing, yet current diagnostic tools used to monitor for the virus rely on slow (>1 h) technologies. Here, we demonstrate the first biosensor capable of detecting the measles virus in minutes with no preprocessing steps. The key sensing element is an electrode coated with a self-assembled monolayer containing the measles antibody, immobilized through an N-heterocyclic carbene (NHC). The intact virus is detected by changes in resistance, giving a linear response to 10-100 mu g/mL of the intact measles virus without the need to label or process the sample. The limit of detection is 6 mu g/mL, which is at the lower limit of concentrations that can cause infections in primates. The NHC-based biosensors are shown to be superior to thiol-based systems, producing an approximately 10x larger response and significantly greater stability toward repeated measurements and long-term storage. This NHC-based biosensor thus represents an important development for both the rapid detection of the measles virus and as a platform technology for the detection of other biological targets of interest.