SEQUENCE ANALYSES OF THE P24 GENE OF BORNA-DISEASE VIRUS IN NATURALLY INFECTED HORSE, DONKEY AND SHEEP
SEQUENCE ANALYSES OF THE P24 GENE OF BORNA-DISEASE VIRUS IN NATURALLY INFECTED HORSE, DONKEY AND SHEEP
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DOI：
10.1016/0168-1702(94)90128-7
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发表时间：
1994-12-01
期刊：
影响因子：
5
通讯作者：
HAGENAU, K
中科院分区：
文献类型：
--
作者：
BINZ, T;LEBELT, J;HAGENAU, K
通过逆转录酶/PCR扩增和p24基因序列测定，确定了博尔纳病病毒（BDV）在不同自然感染动物物种中的亲缘关系。这些结果表明，BDV具有共同的祖先病毒库和非常低的物种屏障。将11个序列与组织培养适应病毒序列进行比较，结果表明，所有分离株在核苷酸水平上同源性至少为96.2%，在氨基酸水平上同源性至少为97%。发现来自羊、驴和马的病毒序列之间的亲缘关系并不比来自不同感染马的病毒序列之间的亲缘关系更远。在一匹马中检测到组织特异性病毒变异：从受感染的大脑和肾脏建立的序列显示10个突变，而从腮腺获得的序列与MDCK细胞适应的BDV的核苷酸序列相比包含20个突变。
By reverse transcriptase/PCR amplification and subsequent sequence determination of the p24 gene, the relatedness of Borna disease virus (BDV) in various naturally infected animal species was determined. These results are indicative of a common ancestral virus pool and a remarkably low species barrier of BDV. Comparison of 11 sequences to that of tissue culture adapted virus revealed that the homology among all isolates was at least 96.2% at the nucleotide level, and 97% at the amino acid level. Viral sequences from sheep, donkey and horse were found to be not more distantly related to each other than sequences from different infected horses. Tissue-specific virus variants were detected in one horse: the sequences established from infected cerebrum and kidney showed 10 mutations, whereas sequences obtained from parotid gland contained 20 mutations in comparison to the nucleotide sequence of MDCK cell adapted BDV.