Chlorophyll Fluorescence Quenching During Photosynthetic Induction in Leaves of Abutilon striatum Dicks. Infected with Abutilon Mosaic Virus, Observed with a Field-Portable Imaging System
Chlorophyll Fluorescence Quenching During Photosynthetic Induction in Leaves of Abutilon striatum Dicks. Infected with Abutilon Mosaic Virus, Observed with a Field-Portable Imaging System
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苘麻纹状体叶片光合诱导过程中叶绿素荧光的猝灭。
DOI：
10.1111/j.1438-8677.1998.tb00724.x
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
U. Lüttge
中科院分区：
文献类型：
--
作者：
C. Osmond;P. Daley;M. Badger;U. Lüttge
:
利用便携式Chi荧光成像系统研究了感染韧皮部定位的abutilon mosaic病毒的<$i荧光猝灭的不均匀性。该仪器被用来观察荧光发射在诱导瞬态期间的间隔，并映射在这些瞬态的过程中施加的饱和脉冲期间的非光化学猝灭。两种症状类型进行了区分：黄色静脉相关的图案，表现出较低的最大Chi荧光比附近的绿色组织，但几乎为零的非光化学淬灭，和静脉定义的马赛克（浅绿色），最初表现出正常的最大Chi荧光，但强烈受损的非光化学淬灭。成熟的静脉定义的马赛克（黄色至白色区域）类似于静脉相关症状，无非光化学猝灭。岛屿明显健康的绿色组织封闭的马赛克症状表现出较慢的非光化学猝灭比对照组。本地化的碳水化合物的积累，被认为是从感染韧皮部限制的病毒，光合作用过程以及叶绿体蛋白质复合物的合成和稳定性的可能影响，在症状发展的背景下进行了讨论。
:
A portable Chi fluorescence imaging system was used to characterise nonuniform Chi fluorescence quenching in Abutilon striatum leaves infected with phloem-localised abutilon mosaic virus. The instrument was used to observe fluorescence emission at intervals during induction transients, and to map nonphotochemical quenching during saturating pulses applied in the course of these transients. Two symptom types were distinguished: yellow vein-associated motifs that showed lower maximum Chi fluorescence than nearby green tissues, but virtually zero nonphotochemical quenching, and vein-defined mosaics (pale green) that initially showed normal maximum Chi fluorescence but strongly impaired nonphotochemical quenching. Mature vein-defined mosaics (yellow to white areas) resembled vein-associated symptoms with zero nonphotochemical quenching. Islands of apparently healthy green tissue enclosed by mosaic symptoms showed slower nonphotochemical quenching than controls. Possible effects of localised carbohydrate accumulation, thought to follow from infection by the phloem-limited virus, on photosynthetic processes as well as the synthesis and stability of chloroplast protein complexes, are discussed in the context of symptom development.