MiR-146b is a regulator of human visceral preadipocyte proliferation and differentiation and its expression is altered in human obesity
MiR-146b is a regulator of human visceral preadipocyte proliferation and differentiation and its expression is altered in human obesity
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MiR-146b 是人内脏前脂肪细胞增殖和分化的调节因子，其表达在人类肥胖中发生改变
DOI：
10.1016/j.mce.2014.05.022
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发表时间：
2014-08-05
影响因子：
4.1
通讯作者：
Guo, Xi-Rong
中科院分区：
文献类型：
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作者：
Chen, Ling;Dai, Yong-Mei;Guo, Xi-Rong
内脏性肥胖是代谢综合征的独立危险因素，异常脂肪积聚与脂肪细胞数量和大小的增加有关。miR-146 b是一种在成熟脂肪细胞中高表达，而在人间充质干细胞（hMSCs）和人内脏前脂肪细胞（vHPA）中低表达的miRNA。本文主要对miR-146 b在脂肪形成中的作用进行了综述。我们发现miR-146 b可以抑制内脏前脂肪细胞的增殖并促进其分化。人内脏脂肪细胞中的miR-146 b抑制KLF 7（Kruppel样转录因子的成员）的表达，如萤火虫荧光素酶报告基因测定所示，表明KLF 7是内源性miR-146 b的直接靶点。在人类超重和肥胖受试者的内脏和皮下脂肪组织中，以及在饮食诱导的肥胖小鼠的附睾脂肪组织和棕色脂肪组织中，miR-146 b表达显著改变。我们的数据表明，miR-146 b可能是一个新的治疗目标，对人类内脏肥胖和代谢功能障碍。(C)2014作者出版社：Elsevier爱尔兰Ltd.
Visceral obesity is an independent risk factor for metabolic syndrome, and abnormal fat accumulation is linked to increases in the number and size of adipocytes. MiR-146b was a miRNA highly expressed in mature adipocytes while very lowly expressed in human mesenchymal stem cells (hMSCs) and human visceral preadipocytes (vHPA). In this paper, we mainly focused on the roles of miR-146b in adipogenesis. We found miR-146b could inhibit the proliferation of visceral preadipocytes and promote their differentiation. MiR-146b in human visceral adipocytes inhibited the expression of KLF7, a member of the Kruppel-like transcription factors, as demonstrated by a firefly luciferase reporter assay, indicating that KLF7 is a direct target of the endogenous miR-146b. MiR-146b expression was significantly altered in visceral and subcutaneous adipose tissues in human overweight and obese subjects, and in the epididymal fat tissues and brown fat tissues of diet-induced obese mice. Our data indicates that miR-146b may be a new therapeutic target against human visceral obesity and metabolic dysfunction. (C) 2014 The Authors. Published by Elsevier Ireland Ltd.