Efficient bioconversion of organic wastes to high optical activity of l-lactic acid stimulated by cathode in mixed microbial consortium.
Efficient bioconversion of organic wastes to high optical activity of l-lactic acid stimulated by cathode in mixed microbial consortium.
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DOI：
10.1016/j.watres.2017.12.024
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发表时间：
2018-03
期刊：
影响因子：
12.8
通讯作者：
G. Xue;Sizhou Lai;Xiang Li;Wenjuan Zhang;Jiguang You;Hong Chen;Yajie Qian;Pin Gao;Zhen-hong Li
中科院分区：
文献类型：
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作者：
G. Xue;Sizhou Lai;Xiang Li;Wenjuan Zhang;Jiguang You;Hong Chen;Yajie Qian;Pin Gao;Zhen-hong Li
乳酸是一种新兴的顶级生物质衍生平台化学品，可以从有机废物中发酵。本研究评价了阴极电发酵（CEF）作为一种利用混合微生物菌群提高有机废弃物中高光学活性（OA）L-乳酸产量的新方法的潜力。与标准氢电极（SHE）相比，通过施加-100 mV的阴极来刺激发酵过程，这有助于提高4.73倍的乳酸生产率（0.6578 g L−1h−1）与开路控制相比（0.1392 g L-1h-1），并且还观察到改善的乳酸的OA（42.3%对开路对照的3.6%）。该研究阐明，与空白相比，-100 mV的最佳电压促进了丙酮酸甲苯-乳酸的转化率达77.9%，这触发了即使在低浓度丙酮酸下也能快速生成L-乳酸。CEF系统中微生物群落在科和属水平上的分布存在显著差异。开路运行试验表明，阴极原位提供电子是实现有机废物高效生物转化为乳酸的必要条件。我们的工作突出了CEF的可行性，引导高附加值的发酵产品来自有机废物的混合微生物财团。
Lactic acid is one of the emerging top biomass derived platform chemicals that can be fermented from organic wastes. This study evaluated the potential of Cathodic Electro-Fermentation (CEF) as a novel approach to enhance the yield of high optical activity (OA) ofl-lactic acid from organic wastes using mixed microbial consortium. The fermentation process was stimulated through the cathode applied with −100 mV versus standard hydrogen electrode (SHE), which contributed to 4.73 times higher lactic acid productivity (0.6578 g L−1h−1) compared to that in the open circuit control (0.1392 g L−1h−1), and an improved OA ofl-lactic acid was also observed (42.3% vs. 3.6% of the open circuit control). The study elucidated that the optimal voltage at −100 mV promoted the conversion of pyruvate tol-lactate by 77.9% compared to the Blank, which triggered the generation ofl-lactic acid to occur rapidly even at low concentration of pyruvate. The significant variation of microbial community in family- and genus-level distributions were observed in CEF system. Furthermore, the open-circuit operation test demonstrated that the cathode providing in-situ electron supply was essential to achieve high efficient bioconversion of organic wastes to lactic acid. Our work highlights the feasibility of CEF to steer high value-added fermentation products deriving from organic wastes by the mixed microbial consortium.