Bdf1p deletion affects mitochondrial function and causes apoptotic cell death under salt stress
Bdf1p deletion affects mitochondrial function and causes apoptotic cell death under salt stress
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Bdf1p 缺失影响线粒体功能并导致盐胁迫下细胞凋亡
DOI：
10.1111/j.1567-1364.2008.00469.x
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发表时间：
2009-03-01
影响因子：
3.2
通讯作者：
Bao, Xiaoming
中科院分区：
文献类型：
--
作者：
Liu, Xiangyong;Yang, Hui;Bao, Xiaoming
酿酒酵母BDF1基因编码含溴结构域的转录因子。我们以前报道过，在酵母细胞中Bdf1p的缺失导致增加对NaCl胁迫的敏感性。在本文中，我们表明Bdf1p在耐盐性中的功能与Ena1p介导的Na+挤出系统以及其他一些特征良好的应激反应途径没有直接联系。有趣的是，然而，我们的数据表明，在NaCl胁迫下，Bdf1p的缺乏导致线粒体功能障碍，包括线粒体膜电位（Δ Psi）降低和活性氧的积累，以及染色质断裂和凝聚。这些结果表明，含溴结构域蛋白，Bdf1p，参与酵母细胞凋亡的调节。
The Saccharomyces cerevisiae BDF1 gene encodes a bromodomain-containing transcription factor. We previously reported that deletion of Bdf1p in yeast cells resulted in increased sensitivity to NaCl stress. In this paper, we show that the function of Bdf1p in salt tolerance is not directly linked with the Ena1p-mediated Na+ extrusion system, and a number of other well-characterized stress-response pathways. Interestingly, however, our data demonstrate that, under the NaCl stress, the absence of Bdf1p leads to mitochondrial dysfunction, including decreasing of mitochondrial membrane potential (Delta Psi) and accumulation of reactive oxygen species, and chromatin fragmentation and condensation. These results indicate that the bromodomain-containing protein, Bdf1p, is involved in the regulation of apoptosis in yeast cells.