Bisphenol-A (BPA) Affects Reproductive Formation across Generations in Mice
Bisphenol-A (BPA) Affects Reproductive Formation across Generations in Mice
复制标题
DOI：
10.1292/jvms.11-0135
复制
发表时间：
2011-09-01
影响因子：
1.2
通讯作者：
Kiso, Yasuo
中科院分区：
文献类型：
--
作者：
Hiyama, Masato;Choi, Ehn-Kyong;Kiso, Yasuo
为了了解双酚A（BPA）暴露对生殖器官的影响，我们分析了第2代子宫和卵巢的形态学以及HOXA 10基因的甲基化模式。妊娠小鼠（F0）在妊娠第12-16天以0- 1，000 mg/kg Bwt的不同剂量SC注射BPA芝麻油溶液。它们的后代（F1）通过寄养小鼠繁殖，并且制备来自F1小鼠的后代（F2）。也就是说，F1小鼠在生殖器官发育期间经历了子宫内BPA暴露，而F2小鼠则完全没有。使用这些F2小鼠，进行本研究。与对照组相比，BPA暴露组的体重显著增加。随着体重的增加，各组卵巢和子宫的相对重量均降低。组织学分析显示子宫腔扩张或堵塞，子宫上皮部分缺失。HOXA 10基因在子宫中的Unroll区域存在非甲基化。本研究提示BPA暴露对F0代小鼠的生殖器官有一定的影响。
To understand effects of Bisphenol-A (BPA) exposure on the reproductive organ across generations, we analyzed morphology of the uterus and ovary, and the methylation pattern of HOXA10 gene of the 2(nd) generation. Pregnant mice (F0) were treated with Sc injection of BPA in sesame oil at various doses of 0-1,000 mg/kg Bwt on days 12-16 of gestation. Their offspring (Fl) were bred by foster mice, and the offspring (F2) from Fl mice were prepared. That is, F1 mice experienced in utero BPA exposure during the developmental period of reproductive organs, while F2 mice did not at all. Using these F2 mice, the present study was carried out. Comparing to the control, the body weights in BPA exposure groups were significantly increased. Correlating with the increase of body weight, the relative weights of the ovary and uterus in each group were decreased. The histological analysis revealed expansion or emphraxis of the uterine lumen and partial loss of the uterine epithelium. Unmethylation of HOXA10 gene in the uterus was observed in the Unroll region. The present study suggested that BPA exposure to F0 mice could affect reproductive organ of F2 mice who were not exposed to BPA.