SEVERAL PATHOGENESIS-RELATED PROTEINS IN POTATO ARE 1,3-BETA-GLUCANASES AND CHITINASES
SEVERAL PATHOGENESIS-RELATED PROTEINS IN POTATO ARE 1,3-BETA-GLUCANASES AND CHITINASES
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DOI：
10.1073/pnas.85.3.782
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发表时间：
1988-02-01
影响因子：
11.1
通讯作者：
HAHLBROCK, K
中科院分区：
文献类型：
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作者：
KOMBRINK, E;SCHRODER, M;HAHLBROCK, K
几丁质酶{（聚[1，4-（N-乙酰基-β- D-氨基葡糖苷）]聚糖水解酶，EC 3.2.1.14}和1，3-β-葡糖苷葡聚糖酶（1，3-β- D-葡聚糖-3-葡聚糖水解酶（EC 3.2.1.6）活性在接种致病真菌致病疫霉（Phytophthora infestans）或用真菌诱导剂处理的马铃薯（Solanum tuberosum）叶片中迅速增加。将酶活性分解为总共两种不同的1，3-β-葡聚糖酶。葡聚糖酶和六种具有几丁质酶活性的蛋白质。根据几个标准，所有这些蛋白质都被归类为“发病相关”蛋白质，其生化功能迄今为止尚不清楚。一些theM构成的主要部分的蛋白质积累在感染马铃薯叶片的细胞间隙中，并被认为在病原体防御中发挥重要作用。
Chitinase {(poly[1,4-(N-acetyl-.beta.-D-glucosaminide)]glycanohydrolase, EC 3.2.1.14} and 1,3-.beta.-glucanase (1,3-.beta.-D-glucan 3-glucanohydrolase, EC 3.2.1.6) activities increased rapidly in potato (Solanum tuberosum) leaves inoculated with the pathogenic fungus Phytophthora infestans or treated with fungal elicitor. The enzyme activities were resolved into a total of two distinct 1,3-.beta.-glucanases and six proteins with chitinase activity. By several criteria, all of these proteins are classified as "pathogenesis-related" proteins whose biochemical functions have so far been unknown. Some of theM constitute a major portion of the proteins accumulating in the intercellular space of infected potato leaves and are assumed to play an important role in pathogen defense.