Determination of starch in plant material
Determination of starch in plant material
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植物材料中淀粉的测定
DOI：
10.1002/jsfa.2740220903
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发表时间：
1971
影响因子：
4.1
通讯作者：
G. N. Richards
中科院分区：
文献类型：
--
作者：
R. Dekker;G. N. Richards
用淀粉-葡萄糖苷酶水解牧草柱花草中的淀粉，然后用葡萄糖氧化酶分析葡萄糖。植物样品中淀粉颗粒在130°C下通过水的预先糊化得到较低的值，并得出结论，淀粉通过碱的完全溶解是必要的，随后用乙酸中和提取物。结果表明，碱也提取出抑制一种或多种参与随后葡萄糖分析的酶的物质，但这种抑制作用可通过用木炭处理提取物而消除。在上述条件下，高乙酸盐浓度降低了最终葡萄糖分析的效率，但这种影响很容易通过使用适当的空白溶液进行校正。与以前的方法相比，所提出的淀粉分析方法在时间上相对经济，并且被认为对低淀粉含量的植物提供更有意义的值。
Starch has been determined in the pasture legume Stylosanthes humilis (Stylo) by hydrolysis with amylo-glucosidase and subsequent analysis for glucose with glucose oxidase. Prior gelatinisation of starch granules within the plant sample by water at 130°c gave low values and it was concluded that the complete solubilisation of starch by alkali was necessary, with subsequent neutralisation of the extract with acetic acid. It was shown that the alkali also extracted material which inhibited one or more of the enzymes involved in the subsequent glucose analysis, but this inhibition was removed by treatment of the extract with charcoal. Under the above conditions the high acetate concentration decreased the efficiency of the final glucose analysis, but this effect was readily corrected by use of appropriate blank solutions. The proposed method of starch analysis is relatively economical in time compared with previous methods and is believed to give more meaningful values for plants with low starch content.
使用葡萄糖氧化酶试剂测定麦芽糖酶和异麦芽糖酶活性。
影响因子：
4.1
作者：
A. Dahlqvist
通讯作者：
A. Dahlqvist