Effect of Adjuvant Release Rate on the Immunogenicity of Nanoparticle-Based Vaccines: A Case Study with a Nanoparticle-Based Nicotine Vaccine.
Effect of Adjuvant Release Rate on the Immunogenicity of Nanoparticle-Based Vaccines: A Case Study with a Nanoparticle-Based Nicotine Vaccine.
复制标题
佐剂释放速率对纳米颗粒疫苗免疫原性的影响：基于纳米颗粒的尼古丁疫苗的案例研究。
DOI：
10.1021/acs.molpharmaceut.9b00279
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发表时间：
2019
影响因子：
4.9
通讯作者：
Zhang,Chenming
中科院分区：
文献类型：
--
作者：
Zhao,Zongmin;Hu,Yun;Harmon,Theresa;Pentel,Paul;Ehrich,Marion;Zhang,Chenming
佐剂是疫苗的关键成分，特别是对于免疫原性差的抗原，如尼古丁。然而，佐剂从疫苗制剂的释放速率对其免疫原性的影响尚未得到很好的说明。在这项研究中，我们制作了一系列的混合纳米粒子为基础的尼古丁疫苗，研究佐剂释放率对其免疫效力的影响。发现具有中等或缓慢佐剂释放速率的纳米疫苗诱导的抗尼古丁抗体滴度显著高于具有快速释放速率的纳米疫苗。此外，在尼古丁激发后，中等和缓慢的佐剂释放速率导致脑尼古丁浓度显著低于快速释放速率。所有研究结果表明，佐剂释放速率影响基于纳米颗粒的尼古丁疫苗的免疫功效，为合理设计针对精神活性药物甚至其他抗原的疫苗制剂提供了潜在的策略。具有优化的佐剂释放速率的基于混合纳米颗粒的尼古丁疫苗可以是有希望的针对尼古丁的下一代免疫候选物。
Adjuvants are a critical component for vaccines, especially for a poorly immunogenic antigen, such as nicotine. However, the impact of adjuvant release rate from a vaccine formulation on its immunogenicity has not been well illustrated. In this study, we fabricated a series of hybrid-nanoparticle-based nicotine vaccines to study the impact of adjuvant release rate on their immunological efficacy. It was found that the nanovaccine with a medium or slow adjuvant release rate induced a significantly higher anti-nicotine antibody titer than that with a fast release rate. Furthermore, the medium and slow adjuvant release rates resulted in a significantly lower brain nicotine concentration than the fast release rate after nicotine challenge. All findings suggest that adjuvant release rate affects the immunological efficacy of nanoparticle-based nicotine vaccines, providing a potential strategy to rationally designing vaccine formulations against psychoactive drugs or even other antigens. The hybrid-nanoparticle-based nicotine vaccine with an optimized adjuvant release rate can be a promising next-generation immunotherapeutic candidate against nicotine.