TGF beta-1 regulation of VEGF production by breast cancer cells
TGF beta-1 regulation of VEGF production by breast cancer cells
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DOI：
10.1007/bf02303745
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发表时间：
1997-12-01
影响因子：
3.7
通讯作者：
BouchierHayes, DJ
中科院分区：
文献类型：
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作者：
Donovan, D;Harmey, JH;BouchierHayes, DJ
背景：血管生成是肿瘤生长和转移的基础。血管内皮生长因子（VEGF）是迄今为止发现的最有效的血管生成因子。方法：采用ELISA法检测乳腺癌患者血清、正常人血清和肿瘤组织中VEGF和TGF β 1的表达。乳腺肿瘤细胞系VEGF蛋白和mRNA的表达进行了检查，并在这些cells中的VEGF生产的TGF β-1的效果进行了assessed.Results：VEGF水平显着较高（P = .03）的乳腺癌患者的血清相比，年龄匹配的对照组。血清VEGF和TGF β 1水平与肿瘤组织VEGF和TGF β 1水平呈正相关（r = 0.539和r = 0.688）。转移性MDA-MB-231乳腺癌细胞比原发性BT474细胞产生更多的VEGF。TGF β 1显著（P
Background: Angiogenesis is essential for tumor growth and metastasis. Vascular endothelial growth factor (VEGF) is the most potent angiogenic factor identified to date. TGF beta-1 acts as an indirect angiogenic agent.Methods: VEGF and TGF beta-1 were measured in the serum of breast cancer patients and age-matched controls and in tumor tissue of cancer patients by ELISA. VEGF protein and mRNA expression by breast tumor cell lines were examined, and the effect of TGF beta-1 on VEGF production in these cells was assessed.Results: VEGF levels were significantly higher (P = .03) in the serum of patients with breast cancer compared to age-matched controls. A positive correlation was found between serum (r = 0.539) and tumor tissue (r = 0.688) levels of VEGF and TGF beta-1. Metastatic MDA-MB-231 breast cancer cells produce more VEGF than do the primary BT474 cells. TGF beta-1 significantly (P