Dynamics of organic‐aggregate‐associated bacterial communities and related environmental factors in Lake Taihu, a large eutrophic shallow lake in China
Dynamics of organic‐aggregate‐associated bacterial communities and related environmental factors in Lake Taihu, a large eutrophic shallow lake in China
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DOI：
10.4319/lo.2010.55.2.0469
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发表时间：
2010-03
影响因子：
4.5
通讯作者：
Xiangming Tang;G. Gao;J. Chao;Xiaodong Wang;G. Zhu;B. Qin
中科院分区：
文献类型：
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作者：
Xiangming Tang;G. Gao;J. Chao;Xiaodong Wang;G. Zhu;B. Qin
为了研究太湖水体中有机聚集体相关细菌群落（OABC）的时空变化，在太湖梅梁湾和湖心两个对照点进行了为期25个月的观测。OABC的结构和动力学通过克隆的16 S rRNA基因的部分测序和末端限制性片段长度多态性（T-RFLP）分析来确定。从两个地点检测到192个T-RF，但只有17个T-RF占优势，这表明特定的微生物种群适应有机聚集体（OA）提供的独特生态位。系统发生学上，β-和α-变形菌构成了数量上最重要的异养细菌群。相似性分析显示，显着的湖内异质性，并在OABC显着的时间变化。典范对应分析表明，沉积物再悬浮和营养盐富集（即硝酸盐和总磷）的站点特定差异是驱动OABC内差异的力量。水温和溶解氧与OABC的季节动态有关。在梅梁湾，OABC的年演替明显，而在湖心则不明显。此外，近35%的细菌序列与来自土壤、活性污泥、沉积物和其他生境的细菌有关，表明外来细菌在OABC中的潜在重要性。
To investigate the spatial and temporal variations of organic‐aggregate‐associated bacterial communities (OABC), a 25‐month observation was conducted at two contrasting sites (Meiliang Bay and the lake center) in Lake Taihu, China. The structures and dynamics of the OABC were determined by partially sequencing cloned 16S rRNA genes, and by terminal restriction fragment length polymorphism (T‐RFLP) analysis. One hundred ninety‐two T‐RFs were detected from the two sites, but only 17 T‐RFs were dominant, suggesting that specific microbial populations were adapted to the unique niche provided by the organic aggregates (OA). Phylogenetically, Beta‐ and Alphaproteobacteria constituted the numerically most important heterotrophic bacterial group. Analysis of similarity revealed significant intra‐lake heterogeneity, and significant temporal shifts in OABC. Canonical correspondence analysis demonstrated that site‐specific differences in sediment resuspension and nutrient enrichment (namely nitrate and total phosphorus) were the forces driving the intra‐lake differences in OABC. Water temperature and dissolved oxygen were related to the seasonal dynamics of OABC. The annual succession in OABC was significant in Meiliang Bay, but not in the lake center. In addition, nearly 35% of the bacterial sequences from OA related to bacteria from soils, activated sludge, sediments, and other habitats, indicating the potential importance of allochthonous bacteria in OABC.